Self-Assembly: Self-Organizing Large-Scale Extracellular-Matrix Protein Networks (Adv. Mater. 18/2015)

2015 ◽  
Vol 27 (18) ◽  
pp. 2813-2813
Author(s):  
Seungkuk Ahn ◽  
Leila F. Deravi ◽  
Sung-Jin Park ◽  
Borna E. Dabiri ◽  
Joon-Seop Kim ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Self Assembly ◽  
Large Scale ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Protein Networks ◽  
Self Organizing

Self-Organizing Large-Scale Extracellular-Matrix Protein Networks

2015 ◽  
Vol 27 (18) ◽  
pp. 2838-2845 ◽  
Author(s):  
Seungkuk Ahn ◽  
Leila F. Deravi ◽  
Sung-Jin Park ◽  
Borna E. Dabiri ◽  
Joon-Seop Kim ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Large Scale ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Protein Networks ◽  
Self Organizing

scholarly journals Self-assembled extracellular matrix protein networks by microcontact printing

Biomaterials ◽  
2004 ◽  
Vol 25 (7-8) ◽  
pp. 1349-1353 ◽  
Author(s):  
Nicoletta Sgarbi ◽  
Dario Pisignano ◽  
Francesca Di Benedetto ◽  
Giuseppe Gigli ◽  
Roberto Cingolani ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Protein ◽  
Microcontact Printing ◽  
Extracellular Matrix Protein ◽  
Protein Networks ◽  
Self Assembled

scholarly journals Crafting of functional biomaterials by directed molecular self-assembly of triple helical peptide building blocks

2017 ◽  
Vol 7 (6) ◽  
pp. 20160138 ◽  
Author(s):  
Jayati Banerjee ◽  
Helena S. Azevedo
Keyword(s):  
Self Assembly ◽  
Large Scale ◽  
Matrix Protein ◽  
Building Blocks ◽  
The Body ◽  
Extracellular Matrix Protein ◽  
Macromolecular Assemblies ◽  
Imino Acid ◽  
Helical Peptide ◽  
Collagen Mimetic Peptides

Collagen is the most abundant extracellular matrix protein in the body and has widespread use in biomedical research, as well as in clinics. In addition to difficulties in the production of recombinant collagen due to its high non-natural imino acid content, animal-derived collagen imposes several major drawbacks—variability in composition, immunogenicity, pathogenicity and difficulty in sequence modification—that may limit its use in the practical scenario. However, in recent years, scientists have shifted their attention towards developing synthetic collagen-like materials from simple collagen model triple helical peptides to eliminate the potential drawbacks. For this purpose, it is highly desirable to develop programmable self-assembling strategies that will initiate the hierarchical self-assembly of short peptides into large-scale macromolecular assemblies with recommendable bioactivity. Herein, we tried to elaborate our understanding related to the strategies that have been adopted by few research groups to trigger self-assembly in the triple helical peptide system producing fascinating supramolecular structures. We have also touched upon the major epitopes within collagen that can be incorporated into collagen mimetic peptides for promoting bioactivity.

1280: Increased Susceptibility to Genitovaginal Prolapse Associated with a Polymorphism in the Promoter of the Extracellular Matrix Protein LAMC1

2007 ◽  
Vol 177 (4S) ◽  
pp. 421-422
Author(s):  
Ganka Nikolova ◽  
Christian O. Twiss ◽  
Hane Lee ◽  
Nelson Stanley ◽  
Janet Sinsheimer ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Increased Susceptibility

scholarly journals Homogenous overexpression of the extracellular matrix protein Netrin-1 in a hollow fiber bioreactor

2021 ◽  
Author(s):  
Aniel Moya-Torres ◽  
Monika Gupta ◽  
Fabian Heide ◽  
Natalie Krahn ◽  
Scott Legare ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Hollow Fiber ◽  
Mammalian Cells ◽  
Matrix Protein ◽  
Continuous Production ◽  
Extracellular Matrix Protein ◽  
Close Monitoring ◽  
High Quality ◽  
Biolayer Interferometry ◽  
Hollow Fiber Bioreactor

Abstract The production of recombinant proteins for functional and biophysical studies, especially in the field of structural determination, still represents a challenge as high quality and quantities are needed to adequately perform experiments. This is in part solved by optimizing protein constructs and expression conditions to maximize the yields in regular flask expression systems. Still, work flow and effort can be substantial with no guarantee to obtain improvements. This study presents a combination of workflows that can be used to dramatically increase protein production and improve processing results, specifically for the extracellular matrix protein Netrin-1. This proteoglycan is an axon guidance cue which interacts with various receptors to initiate downstream signaling cascades affecting cell differentiation, proliferation, metabolism, and survival. We were able to produce large glycoprotein quantities in mammalian cells, which were engineered for protein overexpression and secretion into the media using the controlled environment provided by a hollow fiber bioreactor. Close monitoring of the internal bioreactor conditions allowed for stable production over an extended period of time. In addition to this, Netrin-1 concentrations were monitored in expression media through biolayer interferometry which allowed us to increase Netrin-1 media concentrations tenfold over our current flask systems while preserving excellent protein quality and in solution behavior. Our particular combination of genetic engineering, cell culture system, protein purification, and biophysical characterization permitted us to establish an efficient and continuous production of high-quality protein suitable for structural biology studies that can be translated to various biological systems. Key points • Hollow fiber bioreactor produces substantial yields of homogenous Netrin-1 • Biolayer interferometry allows target protein quantitation in expression media • High production yields in the bioreactor do not impair Netrin-1 proteoglycan quality Graphical abstract

Direct interaction of the extracellular matrix protein DANCE with apolipoprotein(a) mediated by the kringle IV-type 2 domain

2002 ◽  
Vol 267 (4) ◽  
pp. 440-446 ◽  
Author(s):  
A. Kapetanopoulos ◽  
F. Fresser ◽  
G. Millonig ◽  
Y. Shaul ◽  
G. Baier ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Protein ◽  
Direct Interaction ◽  
Extracellular Matrix Protein ◽  
Apolipoprotein A

scholarly journals Characterization of the human extracellular matrix protein 1 gene on chromosome 1q21

1997 ◽  
Vol 16 (5) ◽  
pp. 289-292 ◽  
Author(s):  
Maureen R. Johnson ◽  
Douglas J. Wilkin ◽  
Hans L. Vos ◽  
Rosa Isela Ortiz De Luna ◽  
Anindya M. Dehejia ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Extracellular Matrix Protein 1 ◽  
Chromosome 1Q21
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