scholarly journals Ultraviolet Plasmonic Chirality from Colloidal Aluminum Nanoparticles Exhibiting Charge-Selective Protein Detection

2015 ◽  
Vol 27 (40) ◽  
pp. 6244-6250 ◽  
Author(s):  
Kevin M. McPeak ◽  
Christian D. van Engers ◽  
Sarah Bianchi ◽  
Aurelio Rossinelli ◽  
Lisa V. Poulikakos ◽  
...  
Keyword(s):  
Protein Detection ◽  
Aluminum Nanoparticles ◽  
Colloidal Aluminum

Barcode signal amplifying strategy for sensitive and accurate protein detection on LC-MS/MS

The Analyst ◽  
2021 ◽  
Author(s):  
Chang Shu ◽  
Tengfei Li ◽  
Duo Li ◽  
Zhong-Qiu Li ◽  
Xing-Hua Xia
Keyword(s):  
Side Effects ◽  
Life Science ◽  
Protein Detection ◽  
High Specificity ◽  
Protein Drugs ◽  
P Protein ◽  
Precise Evaluation ◽  
Pharmaceutical Activity ◽  
Low Toxicity

Protein drugs showing strong pharmaceutical activity, high specificity, low toxicity and side effects, have drawn extensive attention from the field of life science and medicine. Precise evaluation of the function...

scholarly journals Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Jie Yun ◽  
Jinyu Ren ◽  
Yufei Liu ◽  
Lijuan Dai ◽  
Liqun Song ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Nephropathy ◽  
High Glucose ◽  
Cell Injury ◽  
Mesangial Cells ◽  
Protein Detection ◽  
Cell Counting ◽  
Luciferase Reporter ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cell Dysfunction

Abstract Background Circular RNAs (circRNAs) have been considered as pivotal biomarkers in Diabetic nephropathy (DN). CircRNA ARP2 actin-related protein 2 homolog (circ-ACTR2) could promote the HG-induced cell injury in DN. However, how circ-ACTR2 acts in DN is still unclear. This study aimed to explore the molecular mechanism of circ-ACTR2 in DN progression, intending to provide support for the diagnostic and therapeutic potentials of circ-ACTR2 in DN. Methods RNA expression analysis was conducted by the quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cell growth was measured via Cell Counting Kit-8 and EdU assays. Inflammatory response was assessed by Enzyme-linked immunosorbent assay. The protein detection was performed via western blot. Oxidative stress was evaluated by the commercial kits. The molecular interaction was affirmed through dual-luciferase reporter and RNA immunoprecipitation assays. Results Circ-ACTR2 level was upregulated in DN samples and high glucose (HG)-treated human renal mesangial cells (HRMCs). Silencing the circ-ACTR2 expression partly abolished the HG-induced cell proliferation, inflammation and extracellular matrix accumulation and oxidative stress in HRMCs. Circ-ACTR2 was confirmed as a sponge for miR-205-5p. Circ-ACTR2 regulated the effects of HG on HRMCs by targeting miR-205-5p. MiR-205-5p directly targeted high-mobility group AT-hook 2 (HMGA2), and HMGA2 downregulation also protected against cell injury in HG-treated HRMCs. HG-mediated cell dysfunction was repressed by miR-205-5p/HMGA2 axis. Moreover, circ-ACTR2 increased the expression of HMGA2 through the sponge effect on miR-205-5p in HG-treated HRMCs. Conclusion All data have manifested that circ-ACTR2 contributed to the HG-induced DN progression in HRMCs by the mediation of miR-205-5p/HMGA2 axis.

Ultra-Trace Protein Detection by Integrating Lateral Flow Biosensor with Ultrasound Enrichment

2021 ◽  
Vol 93 (5) ◽  
pp. 2996-3001
Author(s):  
Yan Huang ◽  
Tailin Xu ◽  
Yong Luo ◽  
Conghui Liu ◽  
Xuan Gao ◽  
...  
Keyword(s):  
Protein Detection ◽  
Lateral Flow ◽  
Ultra Trace

scholarly journals Mass Spectrometry versus Conventional Techniques of Protein Detection: Zika Virus NS3 Protease Activity towards Cellular Proteins

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3732
Author(s):  
Agnieszka Dabrowska ◽  
Aleksandra Milewska ◽  
Joanna Ner-Kluza ◽  
Piotr Suder ◽  
Krzysztof Pyrc
Keyword(s):  
Mass Spectrometry ◽  
Zika Virus ◽  
Viral Infections ◽  
Protein Detection ◽  
Extensive Discussion ◽  
Protein Targets ◽  
Highly Sensitive ◽  
Infected Cells ◽  
Ns3 Protease ◽  
To Receive

Mass spectrometry (MS) used in proteomic approaches is able to detect hundreds of proteins in a single assay. Although undeniable high analytical power of MS, data acquired sometimes lead to confusing results, especially during a search of very selective, unique interactions in complex biological matrices. Here, we would like to show an example of such confusing data, providing an extensive discussion on the observed phenomenon. Our investigations focus on the interaction between the Zika virus NS3 protease, which is essential for virus replication. This enzyme is known for helping to remodel the microenvironment of the infected cells. Several reports show that this protease can process cellular substrates and thereby modify cellular pathways that are important for the virus. Herein, we explored some of the targets of NS3, clearly shown by proteomic techniques, as processed during infection. Unfortunately, we could not confirm the biological relevance of protein targets for viral infections detected by MS. Thus, although mass spectrometry is highly sensitive and useful in many instances, also being able to show directions where cell/virus interaction occurs, we believe that deep recognition of their biological role is essential to receive complete insight into the investigated process.

Biofouling Removal and Protein Detection Using a Hypersonic Resonator

ACS Sensors ◽  
2017 ◽  
Vol 2 (8) ◽  
pp. 1175-1183 ◽  
Author(s):  
Shuting Pan ◽  
Hongxiang Zhang ◽  
Wenpeng Liu ◽  
Yanyan Wang ◽  
Wei Pang ◽  
...  
Keyword(s):  
Protein Detection
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