Labeling of Collagen Type I Templates with a Naturally Derived Contrast Agent for Noninvasive MR Imaging in Soft Tissue Engineering

2018 ◽  
Vol 7 (18) ◽  
pp. 1800605 ◽  
Author(s):  
Heinz P. Janke ◽  
Nihan Güvener ◽  
Weiqiang Dou ◽  
Dorien M. Tiemessen ◽  
Anglita YantiSetiasti ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Mr Imaging ◽  
Contrast Agent ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Soft Tissue Engineering

Fabrication of a RP-Based Biomimetic Grafting Material for Bone Tissue Engineering

2009 ◽  
Vol 626-627 ◽  
pp. 553-558 ◽  
Author(s):  
Xing Ma ◽  
Y.Y. Hu ◽  
Xiao Ming Wu ◽  
J. Liu ◽  
Zhuo Xiong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Collagen Type ◽  
Autologous Bone Marrow ◽  
Collagen Type I ◽  
Control Group ◽  
Type I ◽  
High Porosity ◽  
Highly Porous

Three-dimensional (3D) highly porous poly (DL-lactic-co-glycolic acid)/tricalcium phosphate (PLGA/TCP) scaffolds were fabricated using a rapid prototyping technique (RP). The biopolymer carriers (4mm×4mm×4mm) subsequently were coated with collagen type I (Col) to produce PLGA/TCP/Col composites and utilized as an extracellular matrix for a cell-based strategy of bone tissue engineering. Autologous bone marrow stromal cells (BMSCs) harvested from New Zealand white rabbits were cultured under an osteogenic condition (BMSCs-OB) followed by seeding into the structural highly porous PLGA/TCP/Col composites (i.e. PLGA/TCP/Col/BMSCs-OB). Scanning electron microscopy observation found that the RP-based scaffolds had appropriate microstructure, controlled interconnectivity and high porosity. Modification of the scaffolds with collagen type I (PLGA/TCP/Col) essentially increased the affinity of the carriers to seeding cells, and PLGA/TCP/Col composites were well biocompatible with BMSCs-OB. The PLGA/TCP/Col/BMSCs-OB constructs were then subcutaneously implanted in the back of rabbits compared to controls with autologous BMSCs suspension and carriers alone. As a result, histological new bone formation was observed only in the experimental group with PLGA/TCP/Col/BMSCs-OB constructs 8 weeks after implantation. In the control group with scaffold alone only biodegradation of the carriers was found. Therefore, these results validate our bio-manufacturing methods for a new bone graft substitute.

scholarly journals Ultrarapid Purification of Collagen Type I for Tissue Engineering Applications

2011 ◽  
Vol 17 (9) ◽  
pp. 879-885 ◽  
Author(s):  
Christina A. Pacak ◽  
Jared M. Powers ◽  
Douglas B. Cowan
Keyword(s):  
Tissue Engineering ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Engineering Applications

scholarly journals Characterization of Collagen Type I and II Blended Hydrogels for Articular Cartilage Tissue Engineering

2016 ◽  
Vol 17 (10) ◽  
pp. 3145-3152 ◽  
Author(s):  
Nelda Vázquez-Portalatı́n ◽  
Claire E. Kilmer ◽  
Alyssa Panitch ◽  
Julie C. Liu
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Collagen Type ◽  
Cartilage Tissue Engineering ◽  
Collagen Type I ◽  
Cartilage Tissue ◽  
Type I

scholarly journals Collagen Type I Biomaterials as Scaffolds for Bone Tissue Engineering

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 599
Author(s):  
Gustavo A. Rico-Llanos ◽  
Sara Borrego-González ◽  
Miguelangel Moncayo-Donoso ◽  
José Becerra ◽  
Rick Visser
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Mechanical Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Collagen Type ◽  
Collagen Type I ◽  
Current Status ◽  
Type I ◽  
Organic Constituent

Collagen type I is the main organic constituent of the bone extracellular matrix and has been used for decades as scaffolding material in bone tissue engineering approaches when autografts are not feasible. Polymeric collagen can be easily isolated from various animal sources and can be processed in a great number of ways to manufacture biomaterials in the form of sponges, particles, or hydrogels, among others, for different applications. Despite its great biocompatibility and osteoconductivity, collagen type I also has some drawbacks, such as its high biodegradability, low mechanical strength, and lack of osteoinductive activity. Therefore, many attempts have been made to improve the collagen type I-based implants for bone tissue engineering. This review aims to summarize the current status of collagen type I as a biomaterial for bone tissue engineering, as well as to highlight some of the main efforts that have been made recently towards designing and producing collagen implants to improve bone regeneration.

An improvement of silk-based scaffold properties using collagen type I for skin tissue engineering applications

2017 ◽  
Vol 75 (2) ◽  
pp. 685-700 ◽  
Author(s):  
Suwimon Boonrungsiman ◽  
Nareerat Thongtham ◽  
Orawan Suwantong ◽  
Tuksadon Wutikhun ◽  
Nattakan Soykeabkaew ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Collagen Type ◽  
Collagen Type I ◽  
Skin Tissue ◽  
Type I ◽  
Engineering Applications ◽  
Skin Tissue Engineering ◽  
Scaffold Properties

scholarly journals A 3D-Printed PLCL Scaffold Coated with Collagen Type I and Its Biocompatibility

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Yong He ◽  
Wei Liu ◽  
Lianxiong Guan ◽  
Jielin Chen ◽  
Li Duan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Collagen Type ◽  
Articular Chondrocytes ◽  
Mechanical Test ◽  
Great Influence ◽  
Collagen Type I ◽  
Blue Staining ◽  
Type I ◽  
3D Printed ◽  
Better Than

Scaffolds play an important role in tissue engineering and their structure and biocompatibility have great influence on cell behaviors. In this study, poly(l-lactide-co-ε-caprolactone) (PLCL) scaffolds were printed by a 3D printing technology, low-temperature deposition manufacturing (LDM), and then PLCL scaffolds were treated by alkali and coated with collagen type I (COLI). The scaffolds were characterized by scanning electron microscopy (SEM), porosity test, mechanical test, and infrared spectroscopy. The prepared PLCL and PLCL-COLI scaffolds had three-dimensional (3D) porous structure and they not only have macropores but also have micropores in the deposited lines. Although the mechanical property of PLCL-COLI was slightly lower than that of PLCL scaffold, the hydrophilicity of PLCL-COLI was significantly enhanced. Rabbit articular chondrocytes were extracted and were identified as chondrocytes by toluidine blue staining. To study the biocompatibility, the chondrocytes were seeded on scaffolds for 1, 3, 5, 7, and 10 days. MTT assay showed that the proliferation of chondrocytes on PLCL-COLI scaffold was better than that on PLCL scaffold. And the morphology of cells on PLCL-COLI after 1-day culture was much better than that on PLCL. This 3D-printed PLCL scaffold coated with COLI shows a great potential application in tissue engineering.

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