Pulsed-Electromagnetic-Field-Assisted Reduced Graphene Oxide Substrates for Multidifferentiation of Human Mesenchymal Stem Cells

2016 ◽  
Vol 5 (16) ◽  
pp. 2069-2079 ◽  
Author(s):  
Ki-Taek Lim ◽  
Hoon Seonwoo ◽  
Kyung Soon Choi ◽  
Hexiu Jin ◽  
Kyung-Je Jang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electromagnetic Field ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Human Mesenchymal Stem Cells ◽  
Pulsed Electromagnetic Field ◽  
Reduced Graphene ◽  
Oxide Substrates ◽  
Pulsed Electromagnetic

Reduced graphene oxide-coated hydroxyapatite composites stimulate spontaneous osteogenic differentiation of human mesenchymal stem cells

Nanoscale ◽  
2015 ◽  
Vol 7 (27) ◽  
pp. 11642-11651 ◽  
Author(s):  
Jong Ho Lee ◽  
Yong Cheol Shin ◽  
Oh Seong Jin ◽  
Seok Hee Kang ◽  
Yu-Shik Hwang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Osteogenic Differentiation ◽  
Reduced Graphene Oxide ◽  
Human Mesenchymal Stem Cells ◽  
Reduced Graphene

Reduced graphene oxide-coated hydroxyapatite (rGO-coated HAp) composites stimulated the spontaneous osteogenesis in human mesenchymal stem cells in the absence of osteoinductive agents.

Stimulated Osteogenic Differentiation of Human Mesenchymal Stem Cells by Reduced Graphene Oxide

2015 ◽  
Vol 15 (10) ◽  
pp. 7966-7970 ◽  
Author(s):  
Linhua Jin ◽  
Jong Ho Lee ◽  
Oh Seong Jin ◽  
Yong Cheol Shin ◽  
Min Jeong Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Osteogenic Differentiation ◽  
Reduced Graphene Oxide ◽  
Human Mesenchymal Stem Cells ◽  
Water Soluble ◽  
Alizarin Red ◽  
Reduced Graphene

Osteoprogenitor cells play a significant role in the growth or repair of bones, and have great potential as cell sources for regenerative medicine and bone tissue engineering, but control of their specific differentiation into bone cells remains a challenge. Graphene-based nanomaterials are attractive candidates for biomedical applications as substrates for stem cell (SC) differentiation, scaffolds in tissue engineering, and components of implant devices owing to their biocompatible, transferable and implantable properties. This study examined the enhanced osteogenic differentiation of human mesenchymal stem cells (hMSCs) by reduced graphene oxide (rGO) nanoparticles (NPs), and rGO NPs was prepared by reducing graphene oxide (GO) with a hydrazine treatment followed by annealing in argon and hydrogen. The cytotoxicity profile of each particle was examined using a water-soluble tetrazolium-8 (WST-8) assay. At different time-points, a WST-8 assay, alkaline phosphatase (ALP) activity assay and alizarin red S (ARS) staining were used to determine the effects of rGO NPs on proliferation, differentiation and mineralization, respectively. The results suggest that graphene-based materials have potential as a platform for stem cells culture and biomedicalapplications.

scholarly journals Reduced graphene oxide coating enhances osteogenic differentiation of human mesenchymal stem cells on Ti surfaces

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Moon Sung Kang ◽  
Seung Jo Jeong ◽  
Seok Hyun Lee ◽  
Bongju Kim ◽  
Suck Won Hong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Contact Angle ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Osteogenic Differentiation ◽  
Reduced Graphene Oxide ◽  
Human Mesenchymal Stem Cells ◽  
Alp Activity ◽  
Reduced Graphene ◽  
Graphene Derivatives

Abstract Background Titanium (Ti) has been utilized as hard tissue replacement owing to its superior mechanical and bioinert property, however, lack in tissue compatibility and biofunctionality has limited its clinical use. Reduced graphene oxide (rGO) is one of the graphene derivatives that possess extraordinary biofunctionality and are known to induce osseointegration in vitro and in vivo. In this study, rGO was uniformly coated by meniscus-dragging deposition (MDD) technique to fabricate rGO-Ti substrate for orthopedic and dental implant application. Methods The physicochemical characteristics of rGO-coated Ti (rGO-Ti) substrates were evaluated by atomic force microscopy, water contact angle, and Raman spectroscopy. Furthermore, human mesenchymal stem cells (hMSCs) were cultured on the rGO-Ti substrate, and then their cellular behaviors such as growth and osteogenic differentiation were determined by a cell counting kit-8 assay, alkaline phosphatase (ALP) activity assay, and alizarin red S staining. Results rGO was coated uniformly on Ti substrates by MDD process, which allowed a decrease in the surface roughness and contact angle of Ti substrates. While rGO-Ti substrates significantly increased cell proliferation after 7 days of incubation, they significantly promoted ALP activity and matrix mineralization, which are early and late differentiation markers, respectively. Conclusion It is suggested that rGO-Ti substrates can be effectively utilized as dental and orthopedic bone substitutes since these graphene derivatives have potent effects on stimulating the osteogenic differentiation of hMSCs and showed superior bioactivity and osteogenic potential.

scholarly journals The effect of pulsed electromagnetic field exposure on osteoinduction of human mesenchymal stem cells cultured on nano-TiO2 surfaces

PLoS ONE ◽  
2018 ◽  
Vol 13 (6) ◽  
pp. e0199046 ◽  
Author(s):  
Nora Bloise ◽  
Loredana Petecchia ◽  
Gabriele Ceccarelli ◽  
Lorenzo Fassina ◽  
Cesare Usai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electromagnetic Field ◽  
Human Mesenchymal Stem Cells ◽  
Pulsed Electromagnetic Field ◽  
Nano Tio2 ◽  
Field Exposure ◽  
Pulsed Electromagnetic ◽  
Cells Cultured
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