A Patient-Inspired Ex Vivo Liver Tissue Engineering Approach with Autologous Mesenchymal Stem Cells and Hepatogenic Serum

2016 ◽  
Vol 5 (9) ◽  
pp. 1058-1070 ◽  
Author(s):  
Dillip K. Bishi ◽  
Santosh Mathapati ◽  
Jayarama R. Venugopal ◽  
Soma Guhathakurta ◽  
Kotturathu M. Cherian ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Liver Tissue ◽  
Ex Vivo ◽  
Engineering Approach ◽  
Liver Tissue Engineering ◽  
Tissue Engineering Approach ◽  
Autologous Mesenchymal Stem Cells

Application potential of mesenchymal stem cells derived from Wharton's jelly in liver tissue engineering

2015 ◽  
Vol 25 (s1) ◽  
pp. 137-143 ◽  
Author(s):  
Lei Zhang ◽  
Yong-Hen Zhao ◽  
Zheng Guan ◽  
Jun-Song Ye ◽  
Natalia de Isla ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Liver Tissue ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Liver Tissue Engineering ◽  
Application Potential

Tissue Engineering Approach to the Treatment of Bone Tumors: Three Cases of Cultured Bone Grafts Derived From Patients' Mesenchymal Stem Cells

2006 ◽  
Vol 30 (2) ◽  
pp. 115-118 ◽  
Author(s):  
Toru Morishita ◽  
Kanya Honoki ◽  
Hajime Ohgushi ◽  
Noriko Kotobuki ◽  
Asako Matsushima ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tumors ◽  
Bone Grafts ◽  
Engineering Approach ◽  
Tissue Engineering Approach

TISSUE ENGINEERING APPROACH TO OSTEOCHONDRAL REPAIR AND REGENERATION

2004 ◽  
Vol 04 (04) ◽  
pp. 463-483 ◽  
Author(s):  
J. C. H. GOH ◽  
X. X. SHAO ◽  
D. W. HUTMACHER ◽  
E. H. LEE
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Clinical Medicine ◽  
Osteochondral Lesions ◽  
Fused Deposition ◽  
Engineering Approach ◽  
Osteochondral Scaffold ◽  
Osteochondral Repair ◽  
Tissue Engineering Approach

Repair of osteochondral lesions remains difficult in current clinical medicine. This is due to the lack of self-reparatory capacity in adult cartilage to respond to injuries. Furthermore, current surgical based treatment is unable to achieve long-term satisfactory results. Cell therapies combined with scaffolds has become a promising tissue engineering approach for osteochondral regeneration. This article briefly outlines the approaches and limitations in osteochondral tissue engineering from three key aspects, namely: (1) Cells and Cell Source; (2) Biomaterials and Scaffold design and fabrication; and (3) Mechanical and Biochemical Stimulus. Current optimal candidate cells for tissue engineering include bone marrow and adipose tissue derived mesenchymal stem cells. As for scaffolds, the structural design and biomaterials used should support cell growth and the organization of new functional tissue formation. Using Fused Deposition Modeling (FDM) technique, the authors developed a novel polycaprolactone osteochondral scaffold which was shown to have the ability to recruit mesenchymal stem cells and the potential for repairing defects in vivo. The article also discussed mechanical and biological stimulus for enhancing in vitro growth of tissue-engineered constructs. The final challenge is the integration of the tissue-engineered tissues into a living system as a functional device.

scholarly journals A Tissue Engineering Approach for Periodontal Regeneration Based on a Biodegradable Double-Layer Scaffold and Adipose-Derived Stem Cells

2014 ◽  
Vol 20 (17-18) ◽  
pp. 2483-2492 ◽  
Author(s):  
João F. Requicha ◽  
Carlos A. Viegas ◽  
Fernando Muñoz ◽  
Jorge M. Azevedo ◽  
Isabel B. Leonor ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Double Layer ◽  
Periodontal Regeneration ◽  
Adipose Derived Stem Cells ◽  
Engineering Approach ◽  
Tissue Engineering Approach

The effectiveness of autologous mesenchymal stem cells in the treatment of liver cirrhosis and the method of their visualization in the patient’s body

2020 ◽  
Vol 22 (3) ◽  
pp. 35-40
Author(s):  
I. E. Kotkas ◽  
N. I. Enukashvili ◽  
Sh. M. Asadulayev ◽  
A. V. Chubar’
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Liver Cirrhosis ◽  
Cell Therapy ◽  
Liver Tissue ◽  
Ultrasound Examination ◽  
Test Results ◽  
Resonance Imaging ◽  
Cell Structures ◽  
Autologous Mesenchymal Stem Cells

Abstract. The effectiveness of the influence of autologous mesenchymal stem cells on the function of liver tissue in liver cirrhosis of alimentary etiology is considered, as well as the possibility of visualization of the introduced cell structures with subsequent cytological confirmation. To be able to track autologous mesenchymal stem cells introduced in the patients body, they were labeled with iron oxide nanoparticles. Visualization of the introduced cell structures was performed using magnetic resonance imaging and ultrasound examination of the liver. 6 months after the cell therapy, clinical and biochemical blood tests, lidocaine test results, elastography indicators were evaluated, and the dynamics of hepatic encephalopathy was evaluated. The effectiveness of using autologous mesenchymal stem cells was evidenced by the improvement of the above indicators. Also, 6 months after treatment, a liver tissue biopsy was performed from the sites of fixation of previously introduced cell structures. Histological examination revealed the largest number of labeled cells in the liver micro-nodes, as well as at the borders of micro-nodes and fibrous septa. The use of cell therapy in a patient suffering from liver cirrhosis of alimentary etiology helped to improve the indicators of laboratory and instrumental research methods. No complications were detected during the procedure.

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