Membrane-Directed High Bactericidal Activity of (Gold Nanoparticle)-Polythiophene Composite for Niche Applications Against Pathogenic Bacteria

2012 ◽  
Vol 2 (4) ◽  
pp. 599-606 ◽  
Author(s):  
Manab Deb Adhikari ◽  
Sudeep Goswami ◽  
Biswa Ranjan Panda ◽  
Arun Chattopadhyay ◽  
Aiyagari Ramesh
Keyword(s):  
Gold Nanoparticle ◽  
Bactericidal Activity ◽  
Pathogenic Bacteria

scholarly journals F-type Pyocins are Diverse Non-Contractile Phage Tail-Like Weapons for Killing Pseudomonas aeruginosa

2021 ◽  
Author(s):  
Senjuti Saha ◽  
Chidozie D. Ojobor ◽  
Erik Mackinnon ◽  
Olesia I. North ◽  
Joseph Bondy-Denomy ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bactericidal Activity ◽  
Pathogenic Bacteria ◽  
Therapeutic Potential ◽  
Experimental Studies ◽  
Opportunistic Pathogen ◽  
Multidrug Resistant ◽  
Antibiotic Resistant ◽  
Genes Encoding ◽  
Intraspecies Competition

ABSTRACTMost Pseudomonas aeruginosa strains produce bacteriocins derived from contractile or non-contractile phage tails known as R-type and F-type pyocins, respectively. These bacteriocins possess strain-specific bactericidal activity against P. aeruginosa and likely increase evolutionary fitness through intraspecies competition. R-type pyocins have been studied extensively and show promise as alternatives to antibiotics. Although they have similar therapeutic potential, experimental studies on F-type pyocins are limited. Here, we provide a bioinformatic and experimental investigation of F-type pyocins. We introduce a systematic naming scheme for genes found in R- and F-type pyocin operons and identify 15 genes invariably found in strains producing F-type pyocins. Five proteins encoded at the 3’-end of the F-type pyocin cluster are divergent in sequence, and likely determine bactericidal specificity. We use sequence similarities among these proteins to define 11 distinct F-type pyocin groups, five of which had not been previously described. The five genes encoding the variable proteins associate in two modules that have clearly re-assorted independently during the evolution of these operons. These proteins are considerably more diverse than the specificity-determining tail fibers of R-type pyocins, suggesting that F-type pyocins emerged earlier or have been subject to distinct evolutionary pressures. Experimental studies on six F-type pyocin groups show that each displays a distinct spectrum of bactericidal activity. This activity is strongly influenced by the lipopolysaccharide O-antigen type, but other factors also play a role. F-type pyocins appear to kill as efficiently as R-type pyocins. These studies set the stage for the development of F-type pyocins as anti-bacterial therapeutics.IMPORTANCEPseudomonas aeruginosa is an opportunistic pathogen that causes a broad spectrum of antibiotic resistant infections with high mortality rates, particularly in immunocompromised individuals and cystic fibrosis patients. Due to the increasing frequency of multidrug-resistant P. aeruginosa infections, there is great interest in the development of alternative therapeutics. One alternative is protein-based antimicrobials called bacteriocins, which are produced by one strain of bacteria to kill other strains. In this study, we investigate F-type pyocins, bacteriocins naturally produced by P. aeruginosa that resemble non-contractile phage tails. We show that they are potent killers of P. aeruginosa, and distinct pyocin groups display different killing specificities. We have identified the probable specificity determinants of F-type pyocins, which opens up the potential to engineer them to precisely target strains of pathogenic bacteria. The resemblance of F-type pyocins to well characterized phage tails will greatly facilitate their development into effective antibacterials.

Preparation of graphene oxide (GO)/lanthanum coordination polymers for enhancement of bactericidal activity

2021 ◽  
Author(s):  
Jia Wang ◽  
Zezhi Shan ◽  
Xiao Tan ◽  
Xinxiang Li ◽  
Zhenqi Jiang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Coordination Polymers ◽  
Bactericidal Activity ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Large Scale ◽  
Antibacterial Activities ◽  
Antibacterial Compounds ◽  
Large Scale Preparation ◽  
Scale Preparation

The synthesized GO/lanthanum coordination polymers exhibit broad-spectrum antibacterial activities against seven typical Pathogenic bacteria, are compatible with large-scale preparation and can be harnessed as antibacterial compounds.

Inactivation of Pathogenic Bacteria by Cucumber Volatiles (E,Z)-2,6-Nonadienal and (E)-2-Nonenal†

2004 ◽  
Vol 67 (5) ◽  
pp. 1014-1016 ◽  
Author(s):  
M. J. CHO ◽  
R. W. BUESCHER ◽  
M. JOHNSON ◽  
M. JANES
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Bactericidal Activity ◽  
Pathogenic Bacteria ◽  
Brain Heart Infusion ◽  
Escherichia Coli O157 ◽  
Infusion Solution ◽  
E Coli ◽  
Log Reduction

The effects of (E,Z)-2,6-nonadienal (NDE) and (E)-2-nonenal (NE) on Bacillus cereus, Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella Typhimurium were investigated. A suspension of each organism of 6 to 9 log CFU/ml was incubated for 1 h at 37° C in brain heart infusion solution that contained 0 to 500 or 1,000 ppm of NDE or NE. Depending on concentration, exposure to either NDE or NE caused a reduction in CFU of each organism. Treatment with 250 and 500 ppm NDE completely eliminated viable B. cereus and Salmonella Typhimurium cells, respectively. L. monocytogenes was the most resistant to NDE, showing only about a 2-log reduction from exposure to 500 ppm for 1 h. Conversely, this concentration of NDE caused a 5.8-log reduction in E. coli O157:H7 cells. NE was also effective in inactivating organisms listed above. A higher concentration of NE, 1,000 ppm, was required to kill E. coli O157:H7, L. monocytogenes, or Salmonella Typhimurium compared with NDE. In conclusion, both NDE and NE demonstrated an apparent bactericidal activity against these pathogens.

scholarly journals Bactericidal activity of biosynthesized silver nanoparticles against human pathogenic bacteria

2016 ◽  
Vol 31 (2) ◽  
pp. 411-417 ◽  
Author(s):  
Tarad Abdulaziz Abalkhil ◽  
Sulaiman Ali Alharbi ◽  
Saleh Hussein Salmen ◽  
Milton Wainwright
Keyword(s):  
Silver Nanoparticles ◽  
Bactericidal Activity ◽  
Pathogenic Bacteria ◽  
Human Pathogenic Bacteria

Bactericidal Activity of Different Honeys against Pathogenic Bacteria

2005 ◽  
Vol 36 (5) ◽  
pp. 464-467 ◽  
Author(s):  
Patricia E. Lusby ◽  
Alexandra L. Coombes ◽  
Jenny M. Wilkinson
Keyword(s):  
Bactericidal Activity ◽  
Pathogenic Bacteria

Bioconjugated Gold Nanoparticle for Rapid Capture and Targeted Photothermal Lysis of Pathogenic Bacteria

2012 ◽  
pp. 107-128
Author(s):  
Paresh Chandra Ray ◽  
Sadia Afrin Khan ◽  
Anant Kumar Singh ◽  
Dulal Senapati ◽  
Zhen Fan ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Pathogenic Bacteria

scholarly journals Bacteriostatic and bactericidal activities of Acalypha indica L. plant leave extracts on bacteria shigella disenteriae type 1 in vitro

2021 ◽  
Vol 8 (6) ◽  
pp. 304-309
Author(s):  
Abdul Majid ◽  
Frans Salesman
Keyword(s):  
Experimental Study ◽  
Bactericidal Activity ◽  
Pathogenic Bacteria ◽  
Shigella Dysenteriae ◽  
Herbal Extract ◽  
Bactericidal Activities ◽  
Acalypha Indica ◽  
Shigella Dysenteriae Type

This study aimed to analyze the bacteriostatic and bactericidal activity of Acalypha indica L. extract against Shigella dysenteriae type 1 bacteria in vitro. This research is a laboratory experimental study consisting of six concentration treatments, namely: (K = 0/ml, P1 = 100/ml, P2 = 200/ml, P3 = 400/ml, P4 = 800/ml, P5 = 1600 mg/ml ), and five replications each. The results showed that the herbal extract of Acalypha indica L. was bacteriostatic (inhibiting) at a concentration of 400 mg/mm, and bactericidal (killing) the bacteria Shigella dysenteriae type 1, which was tested in vitro at a concentration of at least 800 mg/ml. It is necessary to study the effect of Acalypha indica L. herbal extract from Timor in the serum of Rattus norwegicus against bacteria that cause bloody diarrhea (EHEC O157:H7, and Shigella dysenteriae type 1), MRSA bacteria, and other pathogenic bacteria.

scholarly journals Elicitation-Based Method for Increasing the Production of Antioxidant and Bactericidal Phenolic Compounds in Dionaea muscipula J. Ellis Tissue

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1794 ◽  
Author(s):  
Wojciech Makowski ◽  
Krzysztof Michał Tokarz ◽  
Barbara Tokarz ◽  
Rafał Banasiuk ◽  
Karolina Witek ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bactericidal Activity ◽  
Pathogenic Bacteria ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Total Phenolic ◽  
Tissue Cultures ◽  
Venus Flytrap ◽  
Dionaea Muscipula ◽  
Plant Culture

The carnivorous plant Dionaea muscipula J. Ellis (Venus flytrap) is a widely known medical herb, capable of producing various phenolic compounds known for their strong antioxidant and antibacterial properties. In the pharmaceutical industry, Venus flytrap is grown in tissue cultures, as the natural population of D. muscipula is very limited. Here, we describe an improved method to increase the quantity and quality of phenolic compounds produced in D. muscipula. This is achieved by combining biotic elicitation (using Cronobacter sakazakii bacteria lysate) of D. muscipula cultured with rotary shaking (hydromechanical stress), which we describe here for the first time. The antibacterial activity and the antioxidant properties of the obtained compounds were studied on two antibiotic-resistant human pathogenic bacteria. The proposed plant culture conditions resulted in an increase in fresh weight, as well as a higher total phenolic content, in comparison to traditional tissue cultures on agar-solidified medium. With the use of high-performance liquid chromatography, we demonstrated that the described elicitation strategy leads to an increased synthesis of myricetin, caffeic acid, ellagic acid and plumbagin in D. muscipula tissue. We also found that a higher level of antioxidant activity, exhibited by the plant extract, corresponded with its higher phenylpropanoid content. The bactericidal activity of the extract against Staphylococcus aureus was dependent on the duration of plant culture under described elicitation conditions, whereas neither elicitation condition (duration or elicitor concentration) seemed relevant for the bactericidal activity of the extract towards Escherichia coli. This suggest that Gram-negative bacteria are less sensitive to compounds derived from Venus flytrap tissue.

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