scholarly journals A Hydrogel Platform that Incorporates Laminin Isoforms for Efficient Presentation of Growth Factors – Neural Growth and Osteogenesis

2021 ◽  
pp. 2010225
Author(s):  
Oana Dobre ◽  
Mariana A. G. Oliva ◽  
Giuseppe Ciccone ◽  
Sara Trujillo ◽  
Aleixandre Rodrigo‐Navarro ◽  
...  
Keyword(s):  
Growth Factors ◽  
Neural Growth

scholarly journals The sulfation of biomimetic glycosaminoglycan substrates controls binding of growth factors and subsequent neural and glial cell growth

2019 ◽  
Vol 7 (10) ◽  
pp. 4283-4298 ◽  
Author(s):  
Waddah Malaeb ◽  
Hisham F. Bahmad ◽  
Wassim Abou-Kheir ◽  
Rami Mhanna
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Cell Growth ◽  
Glial Cell ◽  
Factor Binding ◽  
Control Growth ◽  
Neural Growth

This work shows that alginates can be sulfated to engineer defined substrates that control growth factor binding and neural growth.

Schizophrenia-associated neural growth factors in peripheral blood. A review

2006 ◽  
Vol 16 (7) ◽  
pp. 469-480 ◽  
Author(s):  
Nico J.M. van Beveren ◽  
Job-Jeroen van der Spelt ◽  
Lieuwe de Haan ◽  
Durk Fekkes
Keyword(s):  
Growth Factors ◽  
Peripheral Blood ◽  
Neural Growth

A Non-invasive System for Delivering Neural Growth Factors across the Blood-Brain Barrier: A Review

1998 ◽  
Vol 9 (1) ◽  
Author(s):  
Ann-Charlotte Granholm ◽  
David Albeck ◽  
Cristina Bäckman ◽  
Maria Curtis ◽  
Ted Ebendal ◽  
...  
Keyword(s):  
Growth Factors ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Non Invasive ◽  
Blood Brain ◽  
Neural Growth

scholarly journals Mesenchymal Stromal Cell-Derived Extracellular Vesicles Reduce Neuroinflammation, Promote Neural Cell Proliferation and Improve Oligodendrocyte Maturation in Neonatal Hypoxic-Ischemic Brain Injury

2020 ◽  
Vol 14 ◽  
Author(s):  
Nicole Kaminski ◽  
Christian Köster ◽  
Yanis Mouloud ◽  
Verena Börger ◽  
Ursula Felderhoff-Müser ◽  
...  
Keyword(s):  
Growth Factors ◽  
Brain Injury ◽  
Extracellular Vesicles ◽  
Inflammatory Cytokine ◽  
Therapeutic Effects ◽  
Neonatal Brain ◽  
Promising Alternative ◽  
Neural Growth ◽  
Anti Inflammatory ◽  
The Impact

Background: Neonatal encephalopathy caused by hypoxia-ischemia (HI) is a major cause of childhood mortality and disability. Stem cell-based regenerative therapies seem promising to prevent long-term neurological deficits. Our previous work in neonatal HI revealed an unexpected interaction between mesenchymal stem/stromal cells (MSCs) and the brains' microenvironment leading to an altered therapeutic efficiency. MSCs are supposed to mediate most of their therapeutic effects in a paracrine mode via extracellular vesicles (EVs), which might be an alternative to cell therapy. In the present study, we investigated the impact of MSC-EVs on neonatal HI-induced brain injury.Methods: Nine-day-old C57BL/6 mice were exposed to HI through ligation of the right common carotid artery followed by 1 h hypoxia (10% oxygen). MSC-EVs were injected intraperitoneally 1, 3, and 5 days after HI. One week after HI, brain injury was evaluated by regional neuropathological scoring, atrophy measurements and immunohistochemistry to assess effects on neuronal, oligodendrocyte and vessel densities, proliferation, oligodendrocyte maturation, myelination, astro-, and microglia activation. Immunohistochemistry analyses were complemented by mRNA expression analyses for a broad set of M1/M2- and A1/A2-associated molecules and neural growth factors.Results: While total neuropathological scores and tissue atrophy were not changed, MSC-EVs significantly protected from HI-induced striatal tissue loss and decreased micro- and astroglia activation. MSC-EVs lead to a significant downregulation of the pro-inflammatory cytokine TNFa, accompanied by a significant upregulation of the M2 marker YM-1 and the anti-inflammatory cytokine TGFb. MSC-EVs significantly decreased astrocytic expression of the A1 marker C3, concomitant with an increased expression of neural growth factors (i.e., BDNF, VEGF, and EGF). These alterations were associated with an increased neuronal and vessel density, coinciding with a significant increase of proliferating cells in the neurogenic sub-ventricular zone juxtaposed to the striatum. MSC-EV-mediated neuroprotection went along with a significant improvement of oligodendrocyte maturation and myelination.Conclusion: The present study demonstrates that MSC-EVs mediate anti-inflammatory effects, promote regenerative responses and improve key developmental processes in the injured neonatal brain. The present results suggest different cellular target mechanisms of MSC-EVs, preventing secondary HI-induced brain injury. MSC-EV treatment may be a promising alternative to risk-associated cell therapies in neonatal brain injury.

EARLY PREWEANING METHAMPHETAMINE AND POSTWEANING REARING CONDITIONS INTERFERE WITH THE DEVELOPMENT OF PERIPHERAL STRESS PARAMETERS AND NEURAL GROWTH FACTORS IN GERBILS

2007 ◽  
Vol 117 (11) ◽  
pp. 1621-1638 ◽  
Author(s):  
KONRAD LEHMANN ◽  
ENRIQUE GAREA RODRIGUEZ ◽  
OLIVER KRATZ ◽  
GUNTHER H. MOLL ◽  
RALPH R. DAWIRS ◽  
...  
Keyword(s):  
Growth Factors ◽  
Rearing Conditions ◽  
Neural Growth ◽  
Stress Parameters

scholarly journals Hydrogel Platforms: A Hydrogel Platform that Incorporates Laminin Isoforms for Efficient Presentation of Growth Factors – Neural Growth and Osteogenesis (Adv. Funct. Mater. 21/2021)

2021 ◽  
Vol 31 (21) ◽  
pp. 2170150
Author(s):  
Oana Dobre ◽  
Mariana A. G. Oliva ◽  
Giuseppe Ciccone ◽  
Sara Trujillo ◽  
Aleixandre Rodrigo‐Navarro ◽  
...  
Keyword(s):  
Growth Factors ◽  
Neural Growth

Growth factors and the primary cilium in BALB/c 3T3 cells

1987 ◽  
Vol 45 ◽  
pp. 830-831
Author(s):  
R. W. Tucker ◽  
N. S. More ◽  
S. Jayaraman
Keyword(s):  
Growth Factors ◽  
Primary Cilium ◽  
Cultured Cells ◽  
Morphological Changes ◽  
Calcium Ionophore ◽  
Growth Stimulation ◽  
Calcium Ionophore A23187 ◽  
Ionophore A23187 ◽  
3T3 Cells ◽  
Microtubule Depolymerization

The mechanisms by which polypeptide growth factors Induce DNA synthesis in cultured cells is not understood, but morphological changes Induced by growth factors have been used as clues to Intracellular messengers responsible for growth stimulation. One such morphological change has been the transient disappearance of the primary cilium, a “9 + 0” cilium formed by the perinuclear centriole in interphase cells. Since calcium ionophore A23187 also produced both mitogenesis and ciliary changes, microtubule depolymerization might explain ciliary disappearance monitored by indirect immunofluorescence with anti-tubulin antibody. However, complete resorption and subsequent reformation of the primary cilium occurs at mitosis, and might also account for ciliary disappearance induced by growth factors. To settle this issue, we investigated the ultrastructure of the primary cilium using serial thin-section electron microscopy of quiescent BALB/c 3T3 cells before and after stimulation with serum.

New insights into heparan sulphate biosynthesis from the study of mutant mice

2002 ◽  
Vol 69 ◽  
pp. 47-57 ◽  
Author(s):  
Catherine L. R. Merry ◽  
John T. Gallagher
Keyword(s):  
Growth Factors ◽  
Biosynthetic Pathway ◽  
Heparan Sulphate ◽  
Mutant Mice ◽  
Gene Products ◽  
Multiple Gene ◽  
Adhesion Proteins ◽  
Ligand Interactions

Heparan sulphate (HS) is an essential co-receptor for a number of growth factors, morphogens and adhesion proteins. The biosynthetic modifications involved in the generation of a mature HS chain may determine the strength and outcome of HS–ligand interactions. These modifications are catalysed by a complex family of enzymes, some of which occur as multiple gene products. Various mutant mice have now been generated, which lack the function of isolated components of the HS biosynthetic pathway. In this discussion, we outline the key findings of these studies, and use them to put into context our own work concerning the structure of the HS generated by the Hs2st-/- mice.

Sign in / Sign up

Export Citation Format

Share Document