scholarly journals Void‐Free 3D Bioprinting for In Situ Endothelialization and Microfluidic Perfusion

2019 ◽  
Vol 30 (1) ◽  
pp. 1908349 ◽  
Author(s):  
Liliang Ouyang ◽  
James P. K. Armstrong ◽  
Qu Chen ◽  
Yiyang Lin ◽  
Molly M. Stevens
Keyword(s):  
3D Bioprinting ◽  
Microfluidic Perfusion

scholarly journals 3D-Bioprinting Strategies Based on In Situ Bone-Healing Mechanism for Vascularized Bone Tissue Engineering

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 287
Author(s):  
Ye Lin Park ◽  
Kiwon Park ◽  
Jae Min Cha
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Critical Size ◽  
Current Knowledge ◽  
3D Bioprinting ◽  
The Past ◽  
Regeneration Processes

Over the past decades, a number of bone tissue engineering (BTE) approaches have been developed to address substantial challenges in the management of critical size bone defects. Although the majority of BTE strategies developed in the laboratory have been limited due to lack of clinical relevance in translation, primary prerequisites for the construction of vascularized functional bone grafts have gained confidence owing to the accumulated knowledge of the osteogenic, osteoinductive, and osteoconductive properties of mesenchymal stem cells and bone-relevant biomaterials that reflect bone-healing mechanisms. In this review, we summarize the current knowledge of bone-healing mechanisms focusing on the details that should be embodied in the development of vascularized BTE, and discuss promising strategies based on 3D-bioprinting technologies that efficiently coalesce the abovementioned main features in bone-healing systems, which comprehensively interact during the bone regeneration processes.

scholarly journals Graphene Oxide-Embedded Extracellular Matrix- Derived Hydrogel as a Multiresponsive Platform for 3D Bioprinting Applications

2021 ◽  
Vol 7 (3) ◽  
Author(s):  
Laura Rueda-Gensini ◽  
Julian A Serna ◽  
Javier Cifuentes ◽  
Juan C Cruz ◽  
Carolina Muñoz-Camargo
Keyword(s):  
Graphene Oxide ◽  
Mechanical Stability ◽  
Light Exposure ◽  
Structural Characteristics ◽  
Elastic Response ◽  
Cellular Interaction ◽  
3D Bioprinting ◽  
In Situ Reduction ◽  
Crosslinking Degree

Decellularized extracellular matrices (dECMs) have shown enormous potential for the biofabrication of tissues due to their biomimetic properties that promote enhanced cellular interaction and tissue regeneration. However, biofabrication schemes requiring electrostimulation pose an additional constraint due to the insulating properties of natural materials. Here, we propose a methacryloyl-modified decellularized small intestine submucosa (SISMA) hydrogel, embedded with graphene oxide (GO) nanosheets, for extrusion-based 3D bioprinting applications that require electrostimulation. Methacryloyl biochemicalmodification is performed to enhance the mechanical stability of dECM constructs by mediating photo-crosslinking reactions, and a multistep fabrication scheme is proposed to harness the bioactive and hydrophilic properties of GO and electroconductive properties of reduced GO. For this, GO was initially dispersed in SISMA hydrogels by exploiting its hydrophilicity and protein adsorption capabilities, and in situ reduction was subsequently performed to confer electroconductive abilities. SISMA-GO composite hydrogels were successfully prepared with enhanced structural characteristics, as shown by the higher crosslinking degree and increased elastic response upon blue-light exposure. Moreover, GO was homogeneously dispersed without affecting photocrosslinking reactions and hydrogel shear-thinning properties. Human adipose-derived mesenchymal stem cells were successfully bioprinted in SISMA-GO with high cell viability after 1 week and in situ reduction of GO during this period enhanced the electrical conductivity of these nanostructures. This work demonstrates the potential of SISMA-GO bioinks as bioactive and electroconductive scaffolds for electrostimulation applications in tissue engineering and regenerative medicine.

Paving the path to inpatient printing

2020 ◽  
Vol 12 (551) ◽  
pp. eabd3082
Author(s):  
Ellen Roche
Keyword(s):  
Cell Delivery ◽  
3D Bioprinting ◽  
Live Animal

3D bioprinting in situ in various tissues in a live animal enables in vivo confined cell delivery.

scholarly journals Liver microsystems in vitro for drug response

2019 ◽  
Vol 26 (1) ◽  
Author(s):  
Jyong-Huei Lee ◽  
Kuan-Lun Ho ◽  
Shih-Kang Fan
Keyword(s):  
Soft Lithography ◽  
Drug Response ◽  
Study Drug ◽  
Metabolic Function ◽  
3D Bioprinting ◽  
Cell Micropatterning ◽  
Microfluidic Perfusion ◽  
Parenchymal Cells

Abstract Engineering approaches were adopted for liver microsystems to recapitulate cell arrangements and culture microenvironments in vivo for sensitive, high-throughput and biomimetic drug screening. This review introduces liver microsystems in vitro for drug hepatotoxicity, drug-drug interactions, metabolic function and enzyme induction, based on cell micropatterning, hydrogel biofabrication and microfluidic perfusion. The engineered microsystems provide varied microenvironments for cell culture that feature cell coculture with non-parenchymal cells, in a heterogeneous extracellular matrix and under controllable perfusion. The engineering methods described include cell micropatterning with soft lithography and dielectrophoresis, hydrogel biofabrication with photolithography, micromolding and 3D bioprinting, and microfluidic perfusion with endothelial-like structures and gradient generators. We discuss the major challenges and trends of liver microsystems to study drug response in vitro.

scholarly journals Noninvasive in vivo 3D bioprinting

2020 ◽  
Vol 6 (23) ◽  
pp. eaba7406 ◽  
Author(s):  
Yuwen Chen ◽  
Jiumeng Zhang ◽  
Xuan Liu ◽  
Shuai Wang ◽  
Jie Tao ◽  
...  
Keyword(s):  
3D Printing ◽  
Near Infrared ◽  
Clinical Medicine ◽  
Application Site ◽  
3D Bioprinting ◽  
Printing Technology ◽  
3D Printing Technology ◽  
Tissue Constructs

Three-dimensional (3D) printing technology has great potential in advancing clinical medicine. Currently, the in vivo application strategies for 3D-printed macroscale products are limited to surgical implantation or in situ 3D printing at the exposed trauma, both requiring exposure of the application site. Here, we show a digital near-infrared (NIR) photopolymerization (DNP)–based 3D printing technology that enables the noninvasive in vivo 3D bioprinting of tissue constructs. In this technology, the NIR is modulated into customized pattern by a digital micromirror device, and dynamically projected for spatially inducing the polymerization of monomer solutions. By ex vivo irradiation with the patterned NIR, the subcutaneously injected bioink can be noninvasively printed into customized tissue constructs in situ. Without surgery implantation, a personalized ear-like tissue constructs with chondrification and a muscle tissue repairable cell-laden conformal scaffold were obtained in vivo. This work provides a proof of concept of noninvasive in vivo 3D bioprinting.

scholarly journals Collagen 2A Type B Induction after 3D Bioprinting Chondrocytes In Situ into Osteoarthritic Chondral Tibial Lesion

Cartilage ◽  
2020 ◽  
pp. 194760352090378 ◽  
Author(s):  
Birgitta Gatenholm ◽  
Carl Lindahl ◽  
Mats Brittberg ◽  
Stina Simonsson
Keyword(s):  
Articular Cartilage ◽  
Computer Aided Design ◽  
Tibial Plateau ◽  
Cartilage Tissue ◽  
Patient Specific ◽  
Cartilage Defects ◽  
3D Bioprinting ◽  
Type B ◽  
3D Scanner

Objective Large cartilage defects and osteoarthritis (OA) cause cartilage loss and remain a therapeutic challenge. Three-dimensional (3D) bioprinting with autologous cells using a computer-aided design (CAD) model generated from 3D imaging has the potential to reconstruct patient-specific features that match an articular joint lesion. Design To scan a human OA tibial plateau with a cartilage defect, retrieved after total knee arthroplasty, following clinical imaging techniques were used: (1) computed tomography (CT), (2) magnetic resonance imaging (MRI), and (3) a 3D scanner. From such a scan, a CAD file was obtained to generate G-code to control 3D bioprinting in situ directly into the tibial plateau lesion. Results Highest resolution was obtained using the 3D scanner (2.77 times more points/mm2 than CT), and of the 3 devices tested, only the 3D scanner was able to detect the actual OA defect area. Human chondrocytes included in 3D bioprinted constructs produced extracellular matrix and formed cartilage tissue fragments after 2 weeks of differentiation and high levels of a mature splice version of collagen type II (Col IIA type B), characteristic of native articular cartilage and aggrecan (ACAN). Chondrocytes had a mean viability of 81% in prints after day 5 of differentiation toward cartilage and similar viability was detected in control 3D pellet differentiation of chondrocytes (mean viability 72%). Conclusion Articular cartilage can be formed in 3D bioprints. Thus, this 3D bioprinting system with chondrocytes simulating a patient-specific 3D model provides an attractive strategy for future treatments of cartilage defects or early OA.

scholarly journals 3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissue

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jonathan H. Galarraga ◽  
Mi Y. Kwon ◽  
Jason A. Burdick
Keyword(s):  
Biomedical Applications ◽  
Curing Kinetics ◽  
Cartilage Tissue ◽  
3D Bioprinting ◽  
Capillary Length ◽  
3D Printed ◽  
In Situ Crosslinking ◽  
Printing Parameters ◽  
Histological Staining

Abstract3D bioprinting is a promising approach for the repair of cartilage tissue after damage due to injury or disease; however, the design of 3D printed scaffolds has been limited by the availability of bioinks with requisite printability, cytocompatibility, and bioactivity. To address this, we developed an approach termed in situ crosslinking that permits the printing of non-viscous, photocrosslinkable bioinks via the direct-curing of the bioink with light through a photopermeable capillary prior to deposition. Using a norbornene-modified hyaluronic acid (NorHA) macromer as a representative bioink and our understanding of thiol-ene curing kinetics with visible light, we varied the printing parameters (e.g., capillary length, flow rate, light intensity) to identify printing conditions that were optimal for the ink. The printing process was cytocompatible, with high cell viability and homogenous distribution of mesenchymal stromal cells (MSCs) observed throughout printed constructs. Over 56 days of culture in chondrogenic media, printed constructs increased in compressive moduli, biochemical content (i.e., sulfated glycosaminoglycans, collagen), and histological staining of matrix associated with cartilage tissue. This generalizable printing approach may be used towards the repair of focal defects in articular cartilage or broadly towards widespread biomedical applications across a range of photocrosslinkable bioinks that can now be printed.

Sign in / Sign up

Export Citation Format

Share Document