Tissue Beads: Tissue‐Specific Extracellular Matrix Microbeads to Potentiate Reprogrammed Cell‐Based Therapy

2019 ◽  
Vol 29 (31) ◽  
pp. 1807803 ◽  
Author(s):  
Jung Seung Lee ◽  
Yoon Ho Roh ◽  
Yi Sun Choi ◽  
Yoonhee Jin ◽  
Eun Je Jeon ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Tissue Specific ◽  
Cell Based Therapy

scholarly journals Microbeads: Tissue Beads: Tissue‐Specific Extracellular Matrix Microbeads to Potentiate Reprogrammed Cell‐Based Therapy (Adv. Funct. Mater. 31/2019)

2019 ◽  
Vol 29 (31) ◽  
pp. 1970211
Author(s):  
Jung Seung Lee ◽  
Yoon Ho Roh ◽  
Yi Sun Choi ◽  
Yoonhee Jin ◽  
Eun Je Jeon ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Tissue Specific ◽  
Cell Based Therapy

Effects of adrenocorticotrophic hormone on the expression of matrix metalloproteinases and their inhibitors in the bovine ovary

2020 ◽  
Vol 32 (8) ◽  
pp. 748
Author(s):  
E. M. Belotti ◽  
A. N. Amweg ◽  
V. Matiller ◽  
M. L. Varela ◽  
A. F. Stassi ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Metalloproteinases ◽  
Mrna Expression ◽  
Follicular Fluid ◽  
Adrenocorticotrophic Hormone ◽  
Direct Effects ◽  
Tissue Specific ◽  
Preovulatory Follicles ◽  
Bovine Ovary ◽  
Specific Inhibitors

Cattle undergo numerous environmental and management stressors that reduce fertility and affect ovulation. The extracellular matrix of the follicle wall can be altered by matrix metalloproteinases (MMPs), the activities of which are regulated by interleukins and tissue-specific inhibitors of metalloproteinases (TIMPs), especially during ovulation. The aims of the present study were to: (1) evaluate changes in the hormone milieu, the localisation and activity of MMP2 and MMP9 and the localisation of MMP14, TIMP1 and TIMP2 in response to adrenocorticotrophic hormone (ACTH) during the preovulatory period in cows; and (2) determine the direct effects of ACTH on the mRNA expression of MMP2 and MMP9 in the cultured follicle wall of bovine ovaries obtained from an abattoir. 100IU ACTH was administered during pro-oestrus every 12h until ovariectomy, which was performed before ovulation. Cortisol concentrations in the plasma and follicular fluid (FF) of preovulatory follicles were higher in ACTH-treated than control cows. Progesterone presented subluteal concentrations in plasma of ACTH-treated cows (P<0.05). MMP2 immunostaining and activity in ovaries were higher in ACTH-treated than control cows (P<0.05), whereas MMP9 immunostaining was similar between the two groups. However, unlike in control cows, MMP9 activity was absent in the FF of ACTH-treated cows. These results suggest that the administration of ACTH during the preovulatory period in cows could cause changes that culminate in modifications in the content and activation of MMPs and TIMPs in the ovary, which could interfere with the ovulation process.

scholarly journals Extracellular Matrix Hydrogels Originated from Different Organs Mediate Tissue-Specific Properties and Function

2021 ◽  
Vol 22 (21) ◽  
pp. 11624
Author(s):  
Tzila Davidov ◽  
Yael Efraim ◽  
Rotem Hayam ◽  
Jacopo Oieni ◽  
Limor Baruch ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Pluripotent Stem Cells ◽  
Physical And Mechanical Properties ◽  
Tissue Specific ◽  
Gene Expression Analyses ◽  
Composition Structure ◽  
Induced Pluripotent ◽  
And Function ◽  
First Time

Porcine extracellular matrix (pECM)-derived hydrogels were introduced, in recent years, aiming to benefit the pECM’s microstructure and bioactivity, while controlling the biomaterial’s physical and mechanical properties. The use of pECM from different tissues, however, offers tissue-specific features that can better serve different applications. In this study, pECM hydrogels derived from cardiac, artery, pancreas, and adipose tissues were compared in terms of composition, structure, and mechanical properties. While major similarities were demonstrated between all the pECM hydrogels, their distinctive attributes were also identified, and their substantial effects on cell-ECM interactions were revealed. Furthermore, through comprehensive protein and gene expression analyses, we show, for the first time, that each pECM hydrogel supports the spontaneous differentiation of induced pluripotent stem cells towards the resident cells of its origin tissue. These findings imply that the origin of ECM should be carefully considered when designing a biomedical platform, to achieve a maximal bioactive impact.

scholarly journals Extracellular Matrix Hydrogels Promote Expression of Paxillin, a Muscle-Tendon Junction Marker

2021 ◽  
Author(s):  
Lewis S. Gaffney ◽  
Matthew B. Fisher ◽  
Donald O. Freytes
Keyword(s):  
Extracellular Matrix ◽  
Type I Collagen ◽  
Conditioned Media ◽  
Type I ◽  
Tissue Specific ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions ◽  
Tissue Models ◽  
Cells Cultured

AbstractMuscle and tendon injuries are prevalent and range from minor sprains and strains to traumatic, debilitating injuries. However, the interactions between these tissues during injury and recovery remain unclear. Three-dimensional tissue models that incorporate both tissues and a physiologically relevant junction between muscle and tendon may aide in understanding how the two tissues interact. Here, we use tissue specific extracellular matrix (ECM) derived from muscle and tendon to determine how cells of each tissue interact with the microenvironment of the opposite tissue resulting in junction specific features. ECM materials were derived from the achilles tendon and gastrocnemius muscle, decellularized, and processed to form tissue specific pre-hydrogel digests. C2C12 myoblasts and tendon fibroblasts were cultured in tissue-specific ECM conditioned media or encapsulated in tissue-specific ECM hydrogels to determine cell-matrix interactions and the effects on a muscle-tendon junction marker, paxillin. ECM conditioned media had only a minor effect on upregulation of paxillin in cells cultured in monolayer. However, cells cultured within ECM hydrogels had 50-70% higher paxillin expression than cells cultured in type I collagen hydrogels. Contraction of the ECM hydrogels varied by the type of ECM used. Subsequent experiments with varying density of type I collagen (and thus contraction) showed no correlation between paxillin expression and the amount of gel contraction, suggesting that a constituent of the ECM was the driver of paxillin expression in the ECM hydrogels. Using tissue specific ECM allowed for the de-construction of the cell-matrix interactions similar to muscle-tendon junctions to study the expression of MTJ specific proteins.Impact StatementThe muscle-tendon junction is an important feature of muscle-tendon units; however, despite cross-talk between the two tissue types, it is overlooked in current research. Deconstructing the cell-matrix interactions will allow the opportunity to study significant junction specific features and markers that should be included in tissue models of the muscle-tendon unit, while gaining a deeper understanding of the natural junction. This research aims to inform future methods to engineer a more relevant multi-tissue platform to study the muscle-tendon unit.

scholarly journals Tissue-Specific Effects of Esophageal Extracellular Matrix

2015 ◽  
Vol 21 (17-18) ◽  
pp. 2293-2300 ◽  
Author(s):  
Timothy J. Keane ◽  
Aaron DeWard ◽  
Ricardo Londono ◽  
Lindsey T. Saldin ◽  
Arthur A. Castleton ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Tissue Specific ◽  
Specific Effects
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