Surface State Mediated NIR Two-Photon Fluorescence of Iron Oxides for Nonlinear Optical Microscopy

2012 ◽  
Vol 23 (16) ◽  
pp. 2044-2051 ◽  
Author(s):  
Mei-Yi Liao ◽  
Cheng-Ham Wu ◽  
Ping-Shan Lai ◽  
Jiashing Yu ◽  
Hong-Ping Lin ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Iron Oxides ◽  
Surface State ◽  
Nonlinear Optical ◽  
Nonlinear Optical Microscopy ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Multiple surface plasmon resonances enhanced nonlinear optical microscopy

Nanophotonics ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 487-493 ◽  
Author(s):  
Xiaohu Mi ◽  
Yuyang Wang ◽  
Rui Li ◽  
Mengtao Sun ◽  
Zhenglong Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Optical Microscopy ◽  
Surface Plasmon ◽  
Nonlinear Optical ◽  
Surface Plasmon Resonances ◽  
Nonlinear Optical Microscopy ◽  
Two Photon ◽  
Plasmon Resonances ◽  
Anti Stokes ◽  
Multiple Surface Plasmon Resonances

AbstractThe nonlinear optical microscopies of coherent two-photon excited fluorescence and anti-Stokes Raman scattering are strongly enhanced by multiple surface plasmon resonances (MSPRs). The Au@Ag nanorods presented strong MSPRs peaks at 800 and 400 nm, and can enhance nonlinear optical microscopy at fundamental and double frequencies, respectively. A two-dimensional (2D) material of g-C3N4 is employed to study the plasmon-enhanced nonlinear optical microscopy by the femtosecond laser. The electric analysis reveals that the MSPRs of the Au@Ag nanorod can significantly enhance the signals of two-photon excited fluorescence and anti-Stokes Raman scattering by up to the orders of 104 and 1016, respectively. The results demonstrate the great advantages of plasmon-enhanced nonlinear optical microscopy for the optical analysis on 2D materials, thus providing a new adventure for increasing the optical resolutions of nonlinear optical microscopy.

Aggregation-Induced Nonlinear Optical Effects of AIEgen Nanocrystals for Ultra-Deep in Vivo Bio-Imaging

2019 ◽  
Author(s):  
Zheng Zheng ◽  
Dongyu Li ◽  
zhiyang liu ◽  
Hui-Qing Peng ◽  
Herman H.-Y. Sung ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Spatial Resolution ◽  
Nonlinear Optical ◽  
Near Infrared ◽  
Simple Method ◽  
Nonlinear Optical Microscopy ◽  
Optical Effects ◽  
Nonlinear Optical Effects ◽  
Photon Fluorescence

<p><a></a>Nonlinear optical microscopy has become a powerful tool in bioimaging research due to its unique capabilities of deep optical sectioning, high spatial resolution imaging and three-dimensional reconstruction of biological specimens. Developing organic fluorescent probes with strong nonlinear optical effects, in particular third-harmonic generation (THG), is promising for exploiting nonlinear microscopic imaging for biomedical applications. Herein, we succesfully demonstrate a simple method for preparing organic nanocrystals based on an aggregation-induced emission (AIE) luminogen (DCCN) with bright near-infrared emission. Under femtosecond laser excitation, the high-order nonlinear optical effects of DCCN were studied in three distinct systems, including monomolecules in solution, amorphous nanopaticles, and crystaline nanopaticles. Results revealed aggregation-induced nonlinear optical (AINLO) effects, including two-photon fluorescence (2PF), three-photon fluorescence (3PF) and THG, of DCCN in nanopaticles, especially for the crystaline nanopaticles. Taking advantage of the strong 2PF and THG properties, the nanocrystals of DCCN have been successfully applied for 2PF microscopy at 1040 nm NIR-II excitation and THG microscopy at 1560 nm NIR-II excitation, respectively, to reconstruct the 3D vasculature of the mouse cerebral vasculature. Impressively, the THG microscopy could provide much higher spatial resolution and brightness than the 2PF microscopy and could visualize small vessels with diameters of ~2.7 μm at deepest depth of 800 μm in mouse brain, which is among the largest penetration depth and best spatial resolution of in vivo THG vasculature imaging. Thus, this is expected to inspire new insights into development of advanced AIE materials with multiple nonlinearity, in particular THG, for multimodal nonlinear optical microscopy.<br></p>

A two-photon fluorescence, carbonized polymer dot (CPD)-based, wide range pH nanosensor: a view from the surface state

Nanoscale ◽  
2020 ◽  
Vol 12 (16) ◽  
pp. 9094-9103
Author(s):  
Zepeng Huo ◽  
Gang Chen ◽  
Yijia Geng ◽  
Lili Cong ◽  
Lingyun Pan ◽  
...  
Keyword(s):  
Surface State ◽  
Ph Sensing ◽  
Two Photon ◽  
Wide Range ◽  
Low Toxicity ◽  
Transient Spectroscopy ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A low-toxicity, two-photon pH sensing carbonized polymer dot (CPD) was investigated from a view of surface state by means of the transient spectroscopy.

Aggregation-Induced Nonlinear Optical Effects of AIEgen Nanocrystals for Ultra-Deep in Vivo Bio-Imaging

2019 ◽  
Author(s):  
Zheng Zheng ◽  
Dongyu Li ◽  
zhiyang liu ◽  
Hui-Qing Peng ◽  
Herman H.-Y. Sung ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Spatial Resolution ◽  
Nonlinear Optical ◽  
Near Infrared ◽  
Simple Method ◽  
Nonlinear Optical Microscopy ◽  
Optical Effects ◽  
Nonlinear Optical Effects ◽  
Photon Fluorescence

<p><a></a>Nonlinear optical microscopy has become a powerful tool in bioimaging research due to its unique capabilities of deep optical sectioning, high spatial resolution imaging and three-dimensional reconstruction of biological specimens. Developing organic fluorescent probes with strong nonlinear optical effects, in particular third-harmonic generation (THG), is promising for exploiting nonlinear microscopic imaging for biomedical applications. Herein, we succesfully demonstrate a simple method for preparing organic nanocrystals based on an aggregation-induced emission (AIE) luminogen (DCCN) with bright near-infrared emission. Under femtosecond laser excitation, the high-order nonlinear optical effects of DCCN were studied in three distinct systems, including monomolecules in solution, amorphous nanopaticles, and crystaline nanopaticles. Results revealed aggregation-induced nonlinear optical (AINLO) effects, including two-photon fluorescence (2PF), three-photon fluorescence (3PF) and THG, of DCCN in nanopaticles, especially for the crystaline nanopaticles. Taking advantage of the strong 2PF and THG properties, the nanocrystals of DCCN have been successfully applied for 2PF microscopy at 1040 nm NIR-II excitation and THG microscopy at 1560 nm NIR-II excitation, respectively, to reconstruct the 3D vasculature of the mouse cerebral vasculature. Impressively, the THG microscopy could provide much higher spatial resolution and brightness than the 2PF microscopy and could visualize small vessels with diameters of ~2.7 μm at deepest depth of 800 μm in mouse brain, which is among the largest penetration depth and best spatial resolution of in vivo THG vasculature imaging. Thus, this is expected to inspire new insights into development of advanced AIE materials with multiple nonlinearity, in particular THG, for multimodal nonlinear optical microscopy.<br></p>

scholarly journals Label-free characterization of collagen fibers in cancerous esophagus tissues using ratiometric nonlinear optical microscopy

2020 ◽  
Vol 245 (14) ◽  
pp. 1213-1221
Author(s):  
Wei-Chung Chen ◽  
Yu-Jen Chen ◽  
Shih-Ting Lin ◽  
Wei-Han Hung ◽  
Ming-Che Chan ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Esophageal Cancer ◽  
Esophageal Squamous Cell Carcinoma ◽  
Optical Microscopy ◽  
Harmonic Generation ◽  
Nonlinear Optical ◽  
Second Harmonic ◽  
Collagen Fibers ◽  
Nonlinear Optical Microscopy ◽  
Two Photon

A quantitative analytical method to discriminate among the various types of cancerous esophagus tissue is essential for accurate cancer staging. This paper reports on the use of ratiometric nonlinear optical microscopy to reveal the ratio of two-photon excited fluorescence (TPEF) to second harmonic generation (SHG) and forward to backward (F/B) SHG from single collagen fibers only in submucosa of esophageal squamous cell carcinoma. This makes it possible to accurately differentiate among the four stages of esophageal cancer, providing results that are in good agreement with histopathology. Furthermore, it is confirmed by polarization-dependent SHG that the varied SHG response in esophageal cancer tissues is mainly from the shrinkage in diameter of collagen fibers instead of the collagen triple helixes altered by cancer cells. Based on the results of TPEF/SHG and F/B SHG ratio, they can cooperatively improve the precision of diagnostics on esophageal cancer and could be transferred to other types of cancer diseases with changed collagen fibers. Impact statement The issue of classifying esophageal cancer at various developmental stages is crucial for determining the optimized treatment protocol for the patients, as well as the prognosis. Precision improvement in staging esophageal cancer keeps seeking quantitative and analytical imaging methods that could augment histopathological techniques. In this work, we used nonlinear optical microscopy for ratiometric analysis on the intrinsic signal of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) from single collagen fibers only in submucosa of esophageal squamous cell carcinoma (ESCC). The blind tests of TPEF/SHG and forward (F)/backward (B) SHG were demonstrated to compare with the histology conclusion. The discussion of sensitivity and specificity was provided via statistical comparison between the four stages of esophageal cancer. To the best of our knowledge, this is the first study of using these two ratios in combination for staging ESCC.

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