SERS Aptatags: New Responsive Metallic Nanostructures for Heterogeneous Protein Detection by Surface Enhanced Raman Spectroscopy

2008 ◽  
Vol 18 (17) ◽  
pp. 2518-2525 ◽  
Author(s):  
Laura Fabris ◽  
Mark Dante ◽  
Thuc-Quyen Nguyen ◽  
Jeffrey B.-H. Tok ◽  
Guillermo C. Bazan
Keyword(s):  
Raman Spectroscopy ◽  
Protein Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Metallic Nanostructures ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Low-Copy Number Protein Detection by Electrode Nanogap-Enabled Dielectrophoretic Trapping for Surface-Enhanced Raman Spectroscopy and Electronic Measurements

Nano Letters ◽  
2014 ◽  
Vol 14 (5) ◽  
pp. 2242-2250 ◽  
Author(s):  
Leonardo Lesser-Rojas ◽  
Petra Ebbinghaus ◽  
Ganesh Vasan ◽  
Ming-Lee Chu ◽  
Andreas Erbe ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Copy Number ◽  
Protein Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Low Copy Number ◽  
Surface Enhanced Raman

scholarly journals SARS-CoV-2 Receptor Binding Domain as a Stable-Potential Target for SARS-CoV-2 Detection by Surface—Enhanced Raman Spectroscopy

Sensors ◽  
2021 ◽  
Vol 21 (13) ◽  
pp. 4617
Author(s):  
Chawki Awada ◽  
Mohammed Mahfoudh BA Abdullah ◽  
Hassan Traboulsi ◽  
Chahinez Dab ◽  
Adil Alshoaibi
Keyword(s):  
Raman Spectroscopy ◽  
Detection Limit ◽  
Protein A ◽  
Protein Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Perfect Agreement ◽  
Antibody Recognition ◽  
Surface Enhanced ◽  
Single Protein ◽  
Surface Enhanced Raman

In this work, we report a new approach for detecting SARS-CoV-2 RBD protein (RBD) using the surface-enhanced Raman spectroscopy (SERS) technique. The optical enhancement was obtained thanks to the preparation of nanostructured Ag/Au substrates. Fabricated Au/Ag nanostructures were used in the SERS experiment for RBD protein detection. SERS substrates show higher capabilities and sensitivity to detect RBD protein in a short time (3 s) and with very low power. We were able to push the detection limit of proteins to a single protein detection level of 1 pM. The latter is equivalent to 1 fM as a detection limit of viruses. Additionally, we have shown that the SERS technique was useful to figure out the presence of RBD protein on antibody functionalized substrates. In this case, the SERS detection was based on protein-antibody recognition, which led to shifts in the Raman peaks and allowed signal discrimination between RBD and other targets such as Bovine serum albumin (BSA) protein. A perfect agreement between a 3D simulated model based on finite element method and experiment was reported confirming the SERS frequency shift potential for trace proteins detection. Our results could open the way to develop a new prototype based on SERS sensitivity and selectivity for rapid detection at a very low concentration of virus and even at a single protein level.

Recent Advances in Plasmon-Enhanced Raman Spectroscopies for Catalytic Reactions on Bifunctional Metallic Nanostructures

Nanoscale ◽  
2021 ◽  
Author(s):  
Hai-Sheng Su ◽  
Hui-Shu Feng ◽  
Xiang Wu ◽  
Juan-Juan Sun ◽  
Bin Ren
Keyword(s):  
Raman Spectroscopy ◽  
Reaction Mechanism ◽  
Chemical Reactions ◽  
Surface Enhanced Raman Spectroscopy ◽  
Catalytic Performance ◽  
Metallic Nanostructures ◽  
Catalytic Reactions ◽  
Characterization Methods ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Metallic nanostructures exhibit superior catalytic performance for diverse chemical reactions and the in-depth understanding of reaction mechanism requires versatile characterization methods. Plamon-enhanced Raman spectroscopies (PERS), including surface-enhanced Raman spectroscopy (SERS),...

Aptatag-Based Multiplexed Assay for Protein Detection by Surface-Enhanced Raman Spectroscopy

Small ◽  
2010 ◽  
Vol 6 (14) ◽  
pp. 1550-1557 ◽  
Author(s):  
Laura Fabris ◽  
Martin Schierhorn ◽  
Martin Moskovits ◽  
Guillermo C. Bazan
Keyword(s):  
Raman Spectroscopy ◽  
Protein Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Synthesis and SERS Activity of Core-Satellite Nanoparticles using Heterobifunctional PEG linkers

2021 ◽  
Author(s):  
Angela Michelle San Juan ◽  
Suhash Reddy Chavva ◽  
Dandan Tu ◽  
Melanie Tircuit ◽  
Gerard Cote ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Metallic Nanostructures ◽  
Analytical Technique ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sensitive Analytical Technique

Surface-enhanced Raman spectroscopy (SERS) is a sensitive analytical technique, capable of magnifying the vibrational intensity of a molecule absorbed onto the surface of metallic nanostructures. Various solution-based SERS-active metallic nanostructures...

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