scholarly journals Human In Vitro Model Mimicking Material‐Driven Vascular Regeneration Reveals How Cyclic Stretch and Shear Stress Differentially Modulate Inflammation and Matrix Deposition

2020 ◽  
Vol 4 (6) ◽  
pp. 1900249 ◽  
Author(s):  
Eline E. Haaften ◽  
Tamar B. Wissing ◽  
Nicholas A. Kurniawan ◽  
Anthal I. P. M. Smits ◽  
Carlijn V. C. Bouten
Keyword(s):  
Shear Stress ◽  
In Vitro Model ◽  
Cyclic Stretch ◽  
Matrix Deposition ◽  
Vascular Regeneration ◽  
Vitro Model

Optimization of Schwann Cell Adhesion in Response to Shear Stress in an in Vitro Model for Peripheral Nerve Tissue Engineering

2003 ◽  
Vol 9 (2) ◽  
pp. 233-241 ◽  
Author(s):  
Dara Chafik ◽  
David Bear ◽  
Phong Bui ◽  
Arush Patel ◽  
Neil F. Jones ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Shear Stress ◽  
Cell Adhesion ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
In Vitro Model ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering ◽  
Vitro Model

Endothelial cells in culture: An in vitro model of the wall shear stress gradient effect

1998 ◽  
Vol 31 ◽  
pp. 173 ◽  
Author(s):  
O. Boutherin Falson ◽  
C. Haond ◽  
M. Chaslon ◽  
M. Moenner ◽  
S. Naili ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Wall Shear Stress ◽  
In Vitro Model ◽  
Stress Gradient ◽  
Gradient Effect ◽  
Cells In Culture ◽  
Wall Shear Stress Gradient ◽  
Vitro Model

An in Vitro Model of Aneurysmal Disease: Effect of Leukocyte Infiltration and Shear Stress on MMP Production within the Arterial Wall

1996 ◽  
Vol 800 (1 The Abdominal) ◽  
pp. 270-273 ◽  
Author(s):  
M. M. THOMPSON ◽  
A. WILLS ◽  
E. McDERMOTT ◽  
M. CROWTHER ◽  
N. BRINDLE ◽  
...  
Keyword(s):  
Shear Stress ◽  
Arterial Wall ◽  
In Vitro Model ◽  
Leukocyte Infiltration ◽  
Vitro Model ◽  
Disease Effect

Role Of Cell-Cell Interactions And Cyclic Stretch In An In-Vitro Model Of VALI

2011 ◽  
Author(s):  
Peter C. Stubenrauch ◽  
Erin T. Danielson ◽  
Joshua A. Robertson ◽  
Ann M. Kelly ◽  
Stephanie A. Nonas ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Cell Interactions ◽  
Cyclic Stretch ◽  
Vitro Model ◽  
Cell Cell

scholarly journals Human in vitro model of material-driven vascular regeneration reveals how cyclic stretch and shear stress differentially modulate inflammation and tissue formation

2019 ◽  
Author(s):  
Eline E. van Haaften ◽  
Tamar B. Wissing ◽  
Nicholas A. Kurniawan ◽  
Anthal I.P.M. Smits ◽  
Carlijn V.C. Bouten
Keyword(s):  
Shear Stress ◽  
Tissue Growth ◽  
The Body ◽  
Cyclic Stretch ◽  
Tissue Formation ◽  
Vascular Regeneration ◽  
Underlying Mechanisms ◽  
Living Tissues

1AbstractResorbable synthetic scaffolds designed to regenerate living tissues and organs inside the body emerge as a clinically attractive technology to replace diseased blood vessels. However, mismatches between scaffold design and in vivo hemodynamic loading (i.e., cyclic stretch and shear stress) can result in aberrant inflammation and adverse tissue remodeling, leading to premature graft failure. Yet, the underlying mechanisms remain elusive. Here, a human in vitro model is presented that mimics the transient local inflammatory and biomechanical environments that drive scaffold-guided tissue regeneration. The model is based on the co-culture of human (myo)fibroblasts and macrophages in a bioreactor platform that decouples cyclic stretch and shear stress. Using a resorbable supramolecular elastomer as the scaffold material, it is revealed that cyclic stretch initially reduces pro-inflammatory cytokine secretion and, especially when combined with shear stress, stimulates IL-10 secretion. Moreover, cyclic stretch stimulates downstream (myo)fibroblast proliferation and neotissue formation. In turn, shear stress attenuates cyclic-stretch-induced tissue growth by enhancing MMP-1/TIMP-1-mediated collagen remodeling, and synergistically alters (myo)fibroblast phenotype when combined with cyclic stretch. The findings suggest that shear stress acts as a stabilizing factor in cyclic stretch-induced tissue formation and highlight the distinct roles of hemodynamic loads in the design of resorbable vascular grafts.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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