Comparisons of Stereological and Other Approaches for Quantifying Macrophage Aggregates in Piscine Spleens

2019 ◽  
Vol 31 (4) ◽  
pp. 328-348
Author(s):  
Mark A. Matsche ◽  
Vicki S. Blazer ◽  
Patricia M. Mazik
Keyword(s):  
Macrophage Aggregates

Gonadal macrophage aggregates in fish: A preliminary quantitative study in red mullet

2019 ◽  
Vol 331 (6) ◽  
pp. 357-361
Author(s):  
Valeria Micale ◽  
Anna Perdichizzi ◽  
Ugo Muglia ◽  
Paola Rinelli ◽  
Alessandro Cosenza ◽  
...  
Keyword(s):  
Quantitative Study ◽  
Red Mullet ◽  
Macrophage Aggregates

scholarly journals Enterospora nucleophila (Microsporidia) in Gilthead Sea Bream (Sparus aurata): Pathological Effects and Cellular Immune Response in Natural Infections

2020 ◽  
Vol 57 (4) ◽  
pp. 565-576
Author(s):  
Amparo Picard-Sánchez ◽  
M. Carla Piazzon ◽  
Nahla Hossameldin Ahmed ◽  
Raquel Del Pozo ◽  
Ariadna Sitjà-Bobadilla ◽  
...  
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Sparus Aurata ◽  
Quantitative Polymerase Chain Reaction ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Infection Prevalence ◽  
In Vivo Models ◽  
Cellular Immune ◽  
Macrophage Aggregates

Enterospora nucleophila is a microsporidian responsible for an emaciative disease in gilthead sea bream ( Sparus aurata). Its intranuclear development and the lack of in vitro and in vivo models hinder its research. This study investigated the associated lesions, its detection by quantitative polymerase chain reaction, and the cellular immune response of naturally infected fish. The intensity of infection in the intestine was correlated with stunted growth and reduced body condition. At the beginning of the outbreaks, infection prevalence was highest in intestine and stomach, and in subsequent months, the prevalence decreased in the intestine and increased in hematopoietic organs and stomach. In heavy infections, the intestine had histologic lesions of enterocyte hypercellularity and proliferation of rodlet cells. Infected enterocytes had E. nucleophila spores in the cytoplasm, and a pyknotic nucleus, karyorhexis or karyolysis. Lymphocytes were present at the base of the mucosa, and eosinophilic granule cells were located between the enterocytes. In intestinal submucosa, macrophage aggregates containing spores were surrounded by lymphocytes and granulocytes, with submucosal infiltration of granulocytes. Macrophage aggregates appeared to develop into granulomata with necrotic areas containing parasite remnants. Immunohistochemistry revealed mast cells as the main type of granulocyte involved. Abundant IgM+ and IgT+ cells were identified by in situ hybridization in the submucosa when intracytoplasmic stages were present. This study describes the lesions of E. nucleophila in gilthead sea bream, an important aquaculture species.

Macrophage aggregates in cytomegalovirus esophagitis

1997 ◽  
Vol 28 (3) ◽  
pp. 375-378 ◽  
Author(s):  
Joel K Greenson
Keyword(s):  
Macrophage Aggregates

Chronic Inhalation Toxicity of Fibrous Glass in Rats and Monkeys

1986 ◽  
Vol 5 (6) ◽  
pp. 545-575 ◽  
Author(s):  
R. I. Mitchell ◽  
D. J. Donofrio ◽  
W. J. Moorma
Keyword(s):  
Clinical Signs ◽  
Inhalation Toxicity ◽  
Potential Health ◽  
Inhalation Study ◽  
Pleural Plaques ◽  
Body Weights ◽  
Table Analysis ◽  
F344 Rats ◽  
Macrophage Aggregates

Numerous reports have shown that fibrous glass has the potential to cause fibrogenic and carcinogenic responses in test animals. The experiments producing significant response have, however, used unrealistic routes of exposure. The increased demand for fibrous glass for insulating purposes where respirable fibers may exist presents considerable concern for potential health problems. Therefore, a long-term inhalation study was conducted with F344 rats and cynomolgus monkeys exposed in treatments characterized by fibers of varying geometry and mass concentrations. A workweek type of exposure (7 hours/day, 5 days/week) was maintained for 18 months with monkeys and 21 months with rats, which were subsequently held to 80% mortality. The evaluation of response included life table analysis, body weights, clinical signs, hematological testing, respiratory function, ophthalmic examinations, clinical biochemical analysis, and gross and microscopic pathological examinations. Both species demonstrated pulmonary macrophage aggregates and granulomas containing fibrous glass. The rats had grossly visible pleural plaques, which were not seen in the monkeys. There was no evidence of pulmonary or mesothelial carcinogenicity or fibrogenicity in either species. There were no other significant responses with the exception of a statistically increased mononuclear cell leukemia in each fiber-exposed rat group.

scholarly journals Effects of Unleaded Petroleum on the Macrophage Aggregates (MA) formation in Red King tilapia (Oreochromis sp.) Fingerlings

2016 ◽  
Author(s):  
Ancel Jeff G Beso ◽  
Veronica Y Candelaria ◽  
Jennifer F dela Cruz ◽  
Margie S Tolentino ◽  
Anna Danica C Tameta ◽  
...  
Keyword(s):  
Histological Analysis ◽  
The Philippines ◽  
Splenic Macrophage ◽  
Immune Parameters ◽  
Significant Difference ◽  
Cultured Fish ◽  
The World ◽  
Response To Stress ◽  
Cellular Immune ◽  
Macrophage Aggregates

The Philippines is one of the major producers of tilapia, the most cultured fish and widely consumed in the world. Although fishes in general is said to be adapted to various stressful conditions, the effect on several cellular immune parameters may be of interest to determine the capacity of the organism to withstand stressors. In this paper, the effect of unleaded petroleum on the splenic macrophage aggregate (MA) formation was studied. This was done to have an overview of the immune response of Tilapia or fishes in general when an oil spill, which almost occur annually at different parts of the world, happen. Histological analysis assessed the area occupied by splenic MA 24 hours after introduction of unleaded petroleum to the aquatic system. To determine whether Mabuhay balls, a technology that claims to be beneficial in terms of improving water quality, was added to one tank (T1) to be able to compare it with another tank (T2). There is a strong statistically significant difference between the groups at day1 (p=0.000) opposite the result of day 6 (p=0.155). Thus, unleaded petroleum increased MA formation, a sign that may indicate a high immune activity as an initial positive response to stress. Mabuhay ball have lessen the mortality but has no effect on splenic MA formation.

Sign in / Sign up

Export Citation Format

Share Document