Enzyme Immobilization on Layered and Nanostructured Materials

2010 ◽  
pp. 35-63 ◽  
Author(s):  
Ioannis V. Pavlidis ◽  
Aikaterini A. Tzialla ◽  
Apostolos Enotiadis ◽  
Haralambos Stamatis ◽  
Dimitrios Gournis
Keyword(s):  
Nanostructured Materials ◽  
Enzyme Immobilization

The use of magnetic nanoparticles for immobilization and recycling of enzymes

MRS Advances ◽  
2018 ◽  
Vol 3 (61) ◽  
pp. 3581-3587
Author(s):  
Shirley Furtado ◽  
Mariana Brandes ◽  
Catalina Alamón ◽  
Santiago Botasini ◽  
Ana M.B. Cantera
Keyword(s):  
Magnetic Nanoparticles ◽  
Surface Area ◽  
Nanostructured Materials ◽  
Enzyme Immobilization ◽  
Proteolytic Enzymes ◽  
Large Surface Area ◽  
Emergent Properties ◽  
Initial Activity ◽  
Enzyme Adsorption

ABSTRACTThe use of nanostructured materials for enzyme immobilization is an active field of research due to its large surface area and the new emergent properties derived from its size. The present work is focused on the synthesis of magnetic nanoparticles for the adsorption of cysteine-proteolytic enzymes extracted from Bromelia antiacantha Bertol (Bromeliaceae) fruit. The results show that enzyme adsorption is highly dependent on the temperature and pH. The biocatalyst activity increased up to 40 %, once immobilized onto the magnetic nanoparticles. In addition, they can be recovered using a magnet allowing them to be reused up to 5 cycles with a marginal loss (5 %) of the initial activity.

High-resolution electron microscopy of nanostructured materials

1995 ◽  
Vol 53 ◽  
pp. 172-173
Author(s):  
M. José-Yacamán
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Grain Boundaries ◽  
Nanostructured Materials ◽  
High Resolution Electron Microscopy ◽  
Hrem Image ◽  
Small Particles ◽  
Resolution Electron ◽  
Nanophase Materials

Electron microscopy is a fundamental tool in materials characterization. In the case of nanostructured materials we are looking for features with a size in the nanometer range. Therefore often the conventional TEM techniques are not enough for characterization of nanophases. High Resolution Electron Microscopy (HREM), is a key technique in order to characterize those materials with a resolution of ~ 1.7A. High resolution studies of metallic nanostructured materials has been also reported in the literature. It is concluded that boundaries in nanophase materials are similar in structure to the regular grain boundaries. That work therefore did not confirm the early hipothesis on the field that grain boundaries in nanostructured materials have a special behavior. We will show in this paper that by a combination of HREM image processing, and image calculations, it is possible to prove that small particles and coalesced grains have a significant surface roughness, as well as large internal strain.

Columns: Nanostructured Materials

Polymer News ◽  
2005 ◽  
Vol 30 (7) ◽  
pp. 214-216
Author(s):  
G. Carotenuto
Keyword(s):  
Nanostructured Materials

Column: Nanostructured Materials

Polymer News ◽  
2004 ◽  
Vol 29 (3) ◽  
pp. 77-81
Author(s):  
G. Carotenuto
Keyword(s):  
Nanostructured Materials

Column: Nanostructured Materials

Polymer News ◽  
2004 ◽  
Vol 29 (1) ◽  
pp. 17-18
Author(s):  
G. Carotenuto
Keyword(s):  
Nanostructured Materials

Environmentally Friendly Preparation of Magnetic Composites Featuring Proteolytic Properties

INEOS OPEN ◽  
2020 ◽  
Author(s):  
N. A. Samoilova ◽  
Keyword(s):  
Enzyme Immobilization ◽  
Maleic Acid ◽  
Environmentally Friendly ◽  
Synthetic Polymer ◽  
Magnetic Composites ◽  
Interpolyelectrolyte Complex ◽  
Hydrolysis Rates ◽  
Poly Ethylene ◽  
Noncovalent Binding

The enzyme-containing magnetic composites are presented. The magnetic matrix for enzyme immobilization is obtained by sequential application of an amine-containing polysaccharide—chitosan and a synthetic polymer—poly(ethylene-alt-maleic acid) to the magnetite microparticles to form the interpolyelectrolyte complex shell. Then, the enzyme (trypsin) is immobilized by covalent or noncovalent binding. Thus, the suggested composites can be readily obtained in the environmentally friendly manner. The enzyme capacity of the resulting composites reaches 28.0–32.6 mg/g. The maximum hydrolysis rates of the H-Val-Leu-Lys-pNA substrate provided by these composites range within 0.60·10–7–0.77·10–7 M/min.

Biocatalytic Ketone Reductions Using Biobeads

2020 ◽  
Author(s):  
Jia Shen Chew ◽  
Ken Chi Lik Lee ◽  
THI THANH NHA HO
Keyword(s):  
Enzyme Immobilization ◽  
High Throughput ◽  
High Throughput Screening ◽  
Chiral Hplc ◽  
Micro Scale

<p>Lee and coworkers offers a kind of new concept to enzyme immobilization and explores its suitability in the context of miniaturisation and high-throughput screening. Here, polystyrene-immobilized ketoreductases are compared with its non-immobilized counterparts in terms of conversion and stereoselectivity (both determined by chiral HPLC), and the study indicates that the BioBeads perform similarly (sometimes slightly more selective) which may be useful whenever defined micro-scale amounts of biocatalysts were required in high-throughput experiment settings.</p>

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