Green Sample Preparation with Non-Chromatographic Separation Techniques

2012 ◽  
pp. 125-151 ◽  
Author(s):  
María Dolores Luque de Castro ◽  
Miguel Alcaide Molina
Keyword(s):  
Sample Preparation ◽  
Chromatographic Separation ◽  
Separation Techniques ◽  
Green Sample

scholarly journals PHYTOCHEMICAL SCREENING AND BIOLOGICAL EVALUATION OF DYPSIS LEPTOCHEILOS LEAVES EXTRACT AND MOLECULAR DOCKING STUDY OF THE ISOLATED COMPOUNDS

2020 ◽  
pp. 106-113
Author(s):  
HAITHAM ALI IBRAHIM ◽  
FATEHIA SAYED ELSHARAWY ◽  
MAHMMOUD ELHASSAB ◽  
SAMAH SHABANA ◽  
EMAN GABER HAGGAG
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Chromatographic Separation ◽  
Ethyl Acetate Fraction ◽  
Docking Study ◽  
Separation Techniques ◽  
Reference Drug ◽  
First Time ◽  
Mcf 7

Objective: phytochemical investigation of the ethyl acetate fraction (EAF) of 80% aqueous methanol extract (AME) of Dypsis leptocheilos leaves, in addition to evaluation of the antioxidant, cytotoxic and antimicrobial activities of the AME and EAF. Docking was used to predict and understand cytotoxicity of the isolated compounds. Methods: The ethyl acetate fraction (EAF) of Dypsis leptocheilos leaves was subjected to different chromatographic separation techniques. Structures of the isolated compounds were established by different spectroscopic techniques (1H/13C NMR). Antioxidant activity was evaluated by DPPH assay, while cytotoxicity was evaluated by MTT cell viability assay. Antimicrobial activity was evaluated by agar diffusion method. The docking study was conducted using Auto Dock Vina; the estrogen receptor (PDB 5t92) was used as a receptor for the docking. Results: Chromatographic separation techniques were led to the isolation of five phenolic compounds; these compounds were identified to be apigenin 8-C-β-D-glucopyranoside (Vitexin) (1), apigenin 6-C-β-D-glucopyranoside (Isovitexin) (2), luteolin 7-O-β-D-glucopyranoside (3), luteolin 8-C-β-D-glucopyranoside (Orientin) (4), luteolin 6-C-β-D-glucopyranoside (Isoorientin) (5). They were isolated and identified for the first time from this plant species. The AME and EAF showed moderate activity against Gram positive and Gram negatvie bacteria, while both of them showed similar and powerful antioxidant activity with SC50 = 12.8±0.56 µg/ml and SC50 = 17±0.77 µg/ml respectively, compared to ascorbic (reference drug) SC50 = 14.2±0.35 µg/ml. The EAF showed higher cytotoxic activity on the MCF-7 cells (human breast cancer cell line), with IC50 = 12.3 ± 1.82 µg/ml, compared to Vinblastine Sulfate (reference drug). All isolated compounds showed good binding affinity to the estrogen receptors existed in the MCF-7 cell. Conclusion: Five phenolic compounds were isolated for the first time from the EAF of Dypsis leptocheilos leaves. The AME and EAF extracts showed variable antioxidant, antimicrobial and cytotoxic activities.

Green sample preparation of complex matrices: towards sustainable separations of organic compounds based on the biorefinery concept

2016 ◽  
Vol 88 (1-2) ◽  
pp. 29-36 ◽  
Author(s):  
Vânia G. Zuin
Keyword(s):  
Sample Preparation ◽  
Organic Compounds ◽  
Analytical Techniques ◽  
Complex Matrices ◽  
Extraction And Purification ◽  
Sustainable Extraction ◽  
Green Sample

AbstractThe development and application of green analytical techniques aiming at the sample preparation of complex matrices for the study of organic compounds have been growing considerably over the last 15 years. Miniaturisation, automation and solventless techniques are gaining importance in this field, associated to others, as is the case of metrics. However, the unreflected use of the so-called green analytical techniques “might lead to doing the same things better, rather than rethinking solutions altogether”. Some limits and potentialities of the green sample preparation towards sustainable separations of organic compounds using the biorefinery concept will be also discussed in this paper, a promising biobased route that can integrate sustainable extraction and purification processes in a whole complete circular unity.

Fast chromatographic separation techniques as competitors to chemical and biochemical sensor systems

1994 ◽  
Vol 349 (5) ◽  
pp. 338-345 ◽  
Author(s):  
Karl Cammann ◽  
Wolfgang Kleib�hmer ◽  
Elmar Mussenbrock ◽  
Bernd Ro� ◽  
Frank Zuther
Keyword(s):  
Chromatographic Separation ◽  
Sensor Systems ◽  
Separation Techniques ◽  
Biochemical Sensor

Chromatographic separation techniques and data handling methods for herbal fingerprints: A review

2011 ◽  
Vol 690 (2) ◽  
pp. 148-161 ◽  
Author(s):  
Christophe Tistaert ◽  
Bieke Dejaegher ◽  
Yvan Vander Heyden
Keyword(s):  
Chromatographic Separation ◽  
Data Handling ◽  
Separation Techniques ◽  
Handling Methods

Evaluation of sample preparation and chromatographic separation for the parallel determination of taurine and edaravone in rat tissues using HILIC-MS/MS

2015 ◽  
Vol 407 (14) ◽  
pp. 4143-4153 ◽  
Author(s):  
Yin-jie Li ◽  
Zheng Li ◽  
Xiao-xiao Zheng ◽  
Xiao-wen Wu ◽  
Shi-rui Wang ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Chromatographic Separation ◽  
Rat Tissues
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