Structural and Functional Organization of the Pigment-Protein Complexes of the Photosystems in Mutant Cells of Green Algae and Higher Plants

2015 ◽  
pp. 179-232 ◽  
Author(s):  
Vladimir G. Ladygin
Keyword(s):  
Green Algae ◽  
Functional Organization ◽  
Higher Plants ◽  
Protein Complexes ◽  
Pigment Protein Complexes ◽  
Mutant Cells

Difference picosecond spectroscopy of pigment-protein complexes of photosystem I from higher plants

1984 ◽  
Vol 767 (3) ◽  
pp. 501-506 ◽  
Author(s):  
Marina D. Il'ina ◽  
Vitautas V. Krasauskas ◽  
Richardas J. Rotomskis ◽  
Alexander Yu. Borisov
Keyword(s):  
Photosystem I ◽  
Higher Plants ◽  
Protein Complexes ◽  
Picosecond Spectroscopy ◽  
Pigment Protein Complexes

Occurrence of Secondary Carotenoids in PS I Complexes Isolated from Eremosphaera viridis De Bary (Chlorophyceae)

1992 ◽  
Vol 47 (1-2) ◽  
pp. 51-56 ◽  
Author(s):  
Burkhard Vechtel ◽  
Elfriede K. Pistorius ◽  
Hans Georg Ruppel
Keyword(s):  
Green Algae ◽  
Photosystem I ◽  
Protein Complexes ◽  
Pigment Composition ◽  
Eremosphaera Viridis ◽  
Ps I ◽  
Pigment Protein Complexes ◽  
Secondary Carotenoids ◽  
Lhc I ◽  
Β Carotene

Abstract Photosystem I complexes of Eremosphaera viridis De Bary (Chlorophyceae, Chlorococcales) were isolated and partially characterized. In the isolated PS I complexes, peptides of 64-60, 26, 23, 20, 15, 11 and 8.5 kDa could be detected. When Eremosphaera was grown under regular conditions the pigment composition of the isolated PS I complexes was similar to that found in PS I complexes from other green algae. However, when Eremosphaera was grown under nitrogen deficient conditions, PS I complexes contained the secondary carotenoids canthaxanthin and traces of astaxanthin and echinenone in addition to β-carotene, violaxanthin and lutein. The results presented indicate that the secondary carotenoids are associated with the LHC I of PS I. To our knowledge this represents the first report about the association of secondary carotenoids with light harvesting pigment protein complexes of green algae.

Circular Dichroism of Pigment-Protein Complexes of Higher Plants

1990 ◽  
pp. 1241-1244
Author(s):  
Chong Ci Li ◽  
Xu Chun-Hui ◽  
Dai Yun-Ling ◽  
Zhou Fu-Hong ◽  
Wang Ke-Bin ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Higher Plants ◽  
Protein Complexes ◽  
Pigment Protein Complexes ◽  
Circular Dichroïsm

scholarly journals Photosynthetic pigment-protein complexes optical response modeling optimized by Differential evolution: algorithm convergence study

2021 ◽  
Vol 2090 (1) ◽  
pp. 012028
Author(s):  
Denis D Chesalin ◽  
Roman Y Pishchalnikov
Keyword(s):  
Differential Evolution ◽  
Higher Plants ◽  
Protein Complexes ◽  
Incident Light ◽  
Photosynthetic Pigment ◽  
Differential Evolution Algorithm ◽  
Optimization Procedure ◽  
Optical Response ◽  
Algorithm Convergence ◽  
Pigment Protein Complexes

Abstract Photosynthetic pigment-protein complexes are the essential parts of thylakoid membranes of higher plants and cyanobacteria. Besides many organic and inorganic molecules they contain pigments like chlorophyll, bacteriochlorophyll, and carotenoids, which absorb the incident light and transform it into the energy of the excited electronic states. The semiclassical theories such as molecular exciton theory and the multimode Brownian oscillator model allows us to simulate the linear and nonlinear optical response of any pigment-protein complex, however, the main disadvantage of those approaches is a significant amount of effective parameters needed to be found in order to reproduce the experimental data. To overcome these difficulties we used the Differential evolution method (DE) that belongs to the family of evolutionary optimization algorithms. Based on our preliminary studies of the linear optical properties of monomeric photosynthetic pigments using DE, we proceed to more complex systems like the reaction center of photosystem II isolated from higher plants (PSIIRC). PSIIRC contains only eight chlorophyll pigments, and therefore it is potentially a very promising subject to test DE as a powerful optimization procedure for simulation of the optical response of a system of interacting pigments. Using the theoretically simulated linear spectra of PSIIRC (absorption, circular dichroism, linear dichroism, and fluorescence), we investigated the dependence of the algorithm convergence on DE settings: strategies, crossover, weighting factor; eventually finding the optimal mode of operation of the optimization procedure.

The Occurrence of β-Carotene-5,6-epoxide in the Photosynthetic Apparatus of Higher Plants

1989 ◽  
Vol 44 (11-12) ◽  
pp. 959-965 ◽  
Author(s):  
Andrew Young ◽  
Paul Barry ◽  
George Britton
Keyword(s):  
Higher Plants ◽  
Protein Complexes ◽  
Photosynthetic Apparatus ◽  
Reaction Centre ◽  
Higher Plant ◽  
Ps Ii ◽  
Photooxidative Damage ◽  
Pigment Protein Complexes ◽  
The Individual ◽  
Β Carotene

Abstract The occurrence of β-carotene-5,6-epoxide in higher plant photosynthetic tissue is described. The compound is found in isolated chloroplasts, thylakoids and other subchloroplast particles but can only be detected in intact leaves or cotyledons of higher plants when these are exposed to very high light intensities or to inhibitors such as monuron or paraquat. The distribution of the epoxide within the individual pigment-protein complexes is given. It is particularly associated with the PS I reaction centres (C P I and CP la) and less so with the PS II reaction centre (CPa). Circular dichroism shows that the β-carotene-5,6-epoxide isolated from photosynthetic tissue is optically inactive. It is therefore not produced enzymically but is a product of photooxidative events in the photosynthetic apparatus. Its presence in photosynthetic tissue is a reliable indicator of photooxidative damage to the thylakoid membrane involving oxidation of β-carotene.

scholarly journals Pre-purification of diatom pigment protein complexes provides insight into the heterogeneity of FCP complexes

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Marcel Kansy ◽  
Daniela Volke ◽  
Line Sturm ◽  
Christian Wilhelm ◽  
Ralf Hoffmann ◽  
...  
Keyword(s):  
Spatial Organization ◽  
Higher Plants ◽  
Protein Complexes ◽  
Photosynthetic Apparatus ◽  
Thalassiosira Pseudonana ◽  
Photochemical Quenching ◽  
Complex Nature ◽  
Published Data ◽  
Core Complex ◽  
Pigment Protein Complexes

Abstract Background Although our knowledge about diatom photosynthesis has made huge progress over the last years, many aspects about their photosynthetic apparatus are still enigmatic. According to published data, the spatial organization as well as the biochemical composition of diatom thylakoid membranes is significantly different from that of higher plants. Results In this study the pigment protein complexes of the diatom Thalassiosira pseudonana were isolated by anion exchange chromatography. A step gradient was used for the elution process, yielding five well-separated pigment protein fractions which were characterized in detail. The isolation of photosystem (PS) core complex fractions, which contained fucoxanthin chlorophyll proteins (FCPs), enabled the differentiation between different FCP complexes: FCP complexes which were more closely associated with the PSI and PSII core complexes and FCP complexes which built-up the peripheral antenna. Analysis by mass spectrometry showed that the FCP complexes associated with the PSI and PSII core complexes contained various Lhcf proteins, including Lhcf1, Lhcf2, Lhcf4, Lhcf5, Lhcf6, Lhcf8 and Lhcf9 proteins, while the peripheral FCP complexes were exclusively composed of Lhcf8 and Lhcf9. Lhcr proteins, namely Lhcr1, Lhcr3 and Lhcr14, were identified in fractions containing subunits of the PSI core complex. Lhcx1, Lhcx2 and Lhcx5 proteins co-eluted with PSII protein subunits. The first fraction contained an additional Lhcx protein, Lhcx6_1, and was furthermore characterized by high concentrations of photoprotective xanthophyll cycle pigments. Conclusion The results of the present study corroborate existing data, like the observation of a PSI-specific antenna complex in diatoms composed of Lhcr proteins. They complement other data, like e.g. on the protein composition of the 21 kDa FCP band or the Lhcf composition of FCPa and FCPb complexes. They also provide interesting new information, like the presence of the enzyme diadinoxanthin de-epoxidase in the Lhcx-containing PSII fraction, which might be relevant for the process of non-photochemical quenching. Finally, the high negative charge of the main FCP fraction may play a role in the organization and structure of the native diatom thylakoid membrane. Thus, the results present an important contribution to our understanding of the complex nature of the diatom antenna system.

scholarly journals Photosynthetic pigment-protein complexes as highly connected networks: implications for robust energy transport

2017 ◽  
Vol 473 (2201) ◽  
pp. 20170112 ◽  
Author(s):  
Lewis A. Baker ◽  
Scott Habershon
Keyword(s):  
Energy Transport ◽  
Light Harvesting ◽  
Higher Plants ◽  
Protein Complexes ◽  
Photosynthetic Pigment ◽  
Excitation Energy Transfer ◽  
Reaction Centre ◽  
Lhc Ii ◽  
Pigment Protein Complexes

Photosynthetic pigment-protein complexes (PPCs) are a vital component of the light-harvesting machinery of all plants and photosynthesizing bacteria, enabling efficient transport of the energy of absorbed light towards the reaction centre, where chemical energy storage is initiated. PPCs comprise a set of chromophore molecules, typically bacteriochlorophyll species, held in a well-defined arrangement by a protein scaffold; this relatively rigid distribution leads to a viewpoint in which the chromophore subsystem is treated as a network, where chromophores represent vertices and inter-chromophore electronic couplings represent edges. This graph-based view can then be used as a framework within which to interrogate the role of structural and electronic organization in PPCs. Here, we use this network-based viewpoint to compare excitation energy transfer (EET) dynamics in the light-harvesting complex II (LHC-II) system commonly found in higher plants and the Fenna-Matthews-Olson (FMO) complex found in green sulfur bacteria. The results of our simple network-based investigations clearly demonstrate the role of network connectivity and multiple EET pathways on the efficient and robust EET dynamics in these PPCs, and highlight a role for such considerations in the development of new artificial light-harvesting systems.

Antenna Pigment-Protein Complexes of Higher Plants and Purple Bacteria

1994 ◽  
pp. 55-118 ◽  
Author(s):  
J. Philip Thornber ◽  
Richard J. Cogdell ◽  
Parag Chitnis ◽  
Daryl T. Morishige ◽  
Gary F. Peter ◽  
...  
Keyword(s):  
Higher Plants ◽  
Protein Complexes ◽  
Purple Bacteria ◽  
Pigment Protein Complexes ◽  
Antenna Pigment
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