The Intermediate Lobe of the Pituitary Gland: Introduction and Background

2008 ◽  
pp. 3-12 ◽  
Author(s):  
Aaron B. Lerner
Keyword(s):  
Pituitary Gland ◽  
Intermediate Lobe

The D-2 Dopamine Receptor in the Intermediate Lobe of the Rat Pituitary Gland

1983 ◽  
pp. 33-72 ◽  
Author(s):  
J. W. KEBABIAN ◽  
M. BEAULIEU ◽  
T. E. COTE ◽  
R. L. ESKAY ◽  
E. A. FREY ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Dopamine Receptor ◽  
Intermediate Lobe ◽  
Rat Pituitary

scholarly journals Immunocytochemical demonstration of tissue-type plasminogen activator in endocrine cells of the rat pituitary gland.

1985 ◽  
Vol 101 (1) ◽  
pp. 305-311 ◽  
Author(s):  
P Kristensen ◽  
L S Nielsen ◽  
J Grøndahl-Hansen ◽  
P B Andresen ◽  
L I Larsson ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Pituitary Gland ◽  
Endocrine Cells ◽  
Large Population ◽  
Cell Types ◽  
Anterior Lobe ◽  
Tissue Type ◽  
Intermediate Lobe ◽  
Posterior Lobe ◽  
Rat Pituitary

We immunocytochemically stained rat pituitary glands using antibodies against plasminogen activators of the tissue type (t-PA) and the urokinase type (u-PA). A large population of endocrine cells in the anterior lobe of the gland displayed intense cytoplasmic immunoreactivity with anti-t-PA. In some areas of the intermediate lobe we found a weak staining, and we observed weakly staining granular structures in the posterior lobe. Controls included absorption of the antibodies with highly purified t-PA. In addition, SDS PAGE followed by immunoblotting of pituitary gland extracts revealed only one band with an electrophoretic mobility similar to that of t-PA when stained with anti-t-PA IgG. No u-PA immunoreactivity was detected in the rat pituitary gland. Sequential staining experiments using antibodies against growth hormone and t-PA demonstrated that the t-PA-immunoreactive cells constitute a large subpopulation of the growth hormone-containing cells. These findings represent the first direct evidence for the presence of t-PA in cell types other than endothelial cells in the intact normal organism. In this article we discuss the implications of the results for a possible role of t-PA in the posttranslational processing of prohormones.

Peptides of neuro-intermediate lobe of hog pituitary gland

1957 ◽  
Vol 23 ◽  
pp. 533-540 ◽  
Author(s):  
L.K. Ramachandran ◽  
T. Winnick
Keyword(s):  
Pituitary Gland ◽  
Intermediate Lobe

Bromocriptine-Induced Changes in the Biochemistry, Physiology, and Histology of the Intermediate Lobe of the Rat Pituitary Gland

Endocrinology ◽  
1984 ◽  
Vol 114 (5) ◽  
pp. 1871-1884 ◽  
Author(s):  
M. BEAULIEU ◽  
M. E. GOLDMAN ◽  
K. MIYAZAKI ◽  
E. A. FREY ◽  
R. L. ESKAY ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Intermediate Lobe ◽  
Rat Pituitary ◽  
Induced Changes

Interleukin-1β stimulates cell proliferation in the intermediate lobe of the rat pituitary gland

1994 ◽  
Vol 140 (3) ◽  
pp. 337-341 ◽  
Author(s):  
H Stępień ◽  
G Żerek-Mełen ◽  
S Mucha ◽  
K Winczyk ◽  
J Fryczak
Keyword(s):  
Cell Proliferation ◽  
Cell Growth ◽  
Pituitary Gland ◽  
Molecular Forms ◽  
Brdu Incorporation ◽  
Immunocytochemical Staining ◽  
Pars Intermedia ◽  
Intermediate Lobe ◽  
Intermediate Cell ◽  
Receptor Antibody

Abstract Interleukin-1 (IL-1) is a multifunctional monokine which possesses an impressive array of diverse actions relating to the function of the immune system. IL-1 is present and formed locally in the brain as demonstrated by biochemical and immunocytochemical methods. Various immunomodulatory and neuroendocrine effects of IL-1 have been reported, including induction of several morphological changes in the endocrine cells of experimental animals and humans. IL-1 is present in two molecular forms (IL-1α and IL-1β) that activate specific receptors for IL-1. In the present study we investigated the possible effect of recombinant human IL-1α and IL-1β and recently cloned anti-human IL-1 receptor antibody (M10) on cell proliferation in the anterior and the intermediate lobe of the pituitary gland of the rat. In vivo labelling with bromodeoxyuridine (BrdU) and immunocytochemical staining with anti-BrdU monoclonal antibody were used as a sensitive index of cell proliferation. IL-1β was found to stimulate dose-dependently (0·1–10 μg/kg body weight) incorporation of BrdU into pituitary intermediate cell nuclei, and positive correlation between the tested doses of IL-1β and BrdU-labelling index was noted (r=0·89; P<0·01). This IL-1β-induced stimulation of pituitary pars intermedia cell proliferation was receptor specific, since stimulation was blocked by anti-IL-1 receptor antibody. On the other hand, recombinant human IL-1α did not affect BrdU incorporation and the proliferation of pituitary pars intermedia cells. In addition, neither of the cytokines tested in the same experimental conditions showed any effect on the cell growth of the pituitary pars anterior. These results suggest that IL-1β may be involved in the regulation of the cell growth of the pituitary intermediate lobe in rats. Journal of Endocrinology (1994) 140, 337–341

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