The Effect of Temperature on the Lateral Diffusion of Plasma Membrane Proteins

2008 ◽  
pp. 155-174 ◽  
Author(s):  
Michael Edidin ◽  
Valerie A. Petit
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Lateral Diffusion ◽  
Effect Of Temperature ◽  
Plasma Membrane Proteins

scholarly journals Cell wall constrains lateral diffusion of plant plasma-membrane proteins

2012 ◽  
Vol 109 (31) ◽  
pp. 12805-12810 ◽  
Author(s):  
A. Martiniere ◽  
I. Lavagi ◽  
G. Nageswaran ◽  
D. J. Rolfe ◽  
L. Maneta-Peyret ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Membrane Proteins ◽  
Lateral Diffusion ◽  
Plasma Membrane Proteins ◽  
Plant Plasma Membrane

Detergent fractionation with subsequent subtractive suppression hybridization as a tool for identifying genes coding for plasma membrane proteins

2009 ◽  
Vol 18 (6) ◽  
pp. 527-535 ◽  
Author(s):  
Andreas Lange ◽  
Claudia Kistler ◽  
Tanja B. Jutzi ◽  
Alexandr V. Bazhin ◽  
Claus Detlev Klemke ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Plasma Membrane Proteins ◽  
Subtractive Suppression Hybridization

scholarly journals pH-dependent Cargo Sorting from the Golgi

2011 ◽  
Vol 286 (12) ◽  
pp. 10058-10065 ◽  
Author(s):  
Chunjuan Huang ◽  
Amy Chang
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Atpase Activity ◽  
Secretory Pathway ◽  
Ph Dependence ◽  
Glucose Deprivation ◽  
Ph Homeostasis ◽  
Plasma Membrane Proteins ◽  
Cytosolic Ph ◽  
Cargo Sorting

The vacuolar proton-translocating ATPase (V-ATPase) plays a major role in organelle acidification and works together with other ion transporters to maintain pH homeostasis in eukaryotic cells. We analyzed a requirement for V-ATPase activity in protein trafficking in the yeast secretory pathway. Deficiency of V-ATPase activity caused by subunit deletion or glucose deprivation results in missorting of newly synthesized plasma membrane proteins Pma1 and Can1 directly from the Golgi to the vacuole. Vacuolar mislocalization of Pma1 is dependent on Gga adaptors although no Pma1 ubiquitination was detected. Proper cell surface targeting of Pma1 was rescued in V-ATPase-deficient cells by increasing the pH of the medium, suggesting that missorting is the result of aberrant cytosolic pH. In addition to mislocalization of the plasma membrane proteins, Golgi membrane proteins Kex2 and Vrg4 are also missorted to the vacuole upon loss of V-ATPase activity. Because the missorted cargos have distinct trafficking routes, we suggest a pH dependence for multiple cargo sorting events at the Golgi.

Sign in / Sign up

Export Citation Format

Share Document