Enzyme Reaction Engineering

Biocatalysis ◽  
2005 ◽  
pp. 91-134 ◽  
Keyword(s):  
Enzyme Reaction ◽  
Reaction Engineering

Enzyme Reaction Engineering for Peptide Synthesis

1992 ◽  
Vol 672 (1 Enzyme Engine) ◽  
pp. 528-538 ◽  
Author(s):  
CHRISTIAN WANDREY ◽  
ANDREAS FISCHER ◽  
BURKHARD JOKSCH ◽  
ALEXANDER SCHWARZ
Keyword(s):  
Peptide Synthesis ◽  
Enzyme Reaction ◽  
Reaction Engineering

Enzyme reaction engineering: Design of peptide synthesis by stabilized trypsin

1991 ◽  
Vol 13 (7) ◽  
pp. 573-583 ◽  
Author(s):  
Rosa M. Blanco ◽  
Gregorio Alvaro ◽  
JoséM. Guisán
Keyword(s):  
Engineering Design ◽  
Peptide Synthesis ◽  
Enzyme Reaction ◽  
Reaction Engineering

ChemInform Abstract: Enzyme Reaction Engineering

ChemInform ◽  
2010 ◽  
Vol 24 (32) ◽  
pp. no-no
Author(s):  
C. WANDREY
Keyword(s):  
Enzyme Reaction ◽  
Reaction Engineering

Modelling as a tool of enzyme reaction engineering for enzyme reactor development

2011 ◽  
Vol 91 (4) ◽  
pp. 845-856 ◽  
Author(s):  
Durda Vasić-Rački ◽  
Zvjezdana Findrik ◽  
Ana Vrsalović Presečki
Keyword(s):  
Enzyme Reaction ◽  
Reaction Engineering ◽  
Enzyme Reactor

Lead aspartate: a new en bloc stain for electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 34-35
Author(s):  
J.R. Walton
Keyword(s):  
Electron Microscopy ◽  
Calcium Ion ◽  
Enzyme Reaction ◽  
Lead Citrate ◽  
Reaction Products ◽  
En Bloc ◽  
Thin Sections ◽  
Lead Salts ◽  
Thin Sectioning ◽  
High Alkalinity

In electron microscopy, lead is the metal most widely used for enhancing specimen contrast. Lead citrate requires a pH of 12 to stain thin sections of epoxy-embedded material rapidly and intensively. However, this high alkalinity tends to leach out enzyme reaction products, making lead citrate unsuitable for many cytochemical studies. Substitution of the chelator aspartate for citrate allows staining to be carried out at pH 6 or 7 without apparent effect on cytochemical products. Moreover, due to the low, controlled level of free lead ions, contamination-free staining can be carried out en bloc, prior to dehydration and embedding. En bloc use of lead aspartate permits the grid-staining step to be bypassed, allowing samples to be examined immediately after thin-sectioning.Procedures. To prevent precipitation of lead salts, double- or glass-distilled H20 used in the stain and rinses should be boiled to drive off carbon dioxide and glassware should be carefully rinsed to remove any persisting traces of calcium ion.

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