Investigation of Phage Display for the Directed Evolution of Enzymes

2003 ◽  
pp. 79-110 ◽  
Author(s):  
Patrice Soumillion ◽  
Jacques Fastrez
Keyword(s):  
Phage Display ◽  
Directed Evolution

scholarly journals A substrate selected by phage display exhibits enhanced side-chain hydrogen bonding to HIV-1 protease

2018 ◽  
Vol 74 (7) ◽  
pp. 690-694 ◽  
Author(s):  
Ian W. Windsor ◽  
Ronald T. Raines
Keyword(s):  
Crystal Structure ◽  
Hydrogen Bonding ◽  
Molecular Recognition ◽  
Hydrogen Bonds ◽  
Phage Display ◽  
Directed Evolution ◽  
Side Chain ◽  
Endogenous Substrates ◽  
Hiv 1 ◽  
Additional Hydrogen

Crystal structures of inactive variants of HIV-1 protease bound to peptides have revealed how the enzyme recognizes its endogenous substrates. The best of the known substrates is, however, a nonnatural substrate that was identified by directed evolution. The crystal structure of the complex between this substrate and the D25N variant of the protease is reported at a resolution of 1.1 Å. The structure has several unprecedented features, especially the formation of additional hydrogen bonds between the enzyme and the substrate. This work expands the understanding of molecular recognition by HIV-1 protease and informs the design of new substrates and inhibitors.

A strategy for the generation of specific human antibodies by directed evolution and phage display

FEBS Journal ◽  
2005 ◽  
Vol 272 (10) ◽  
pp. 2591-2601 ◽  
Author(s):  
Lidia Riaño-Umbarila ◽  
Victor Rivelino Juárez-González ◽  
Timoteo Olamendi-Portugal ◽  
Mauricio Ortíz-León ◽  
Lourival Domingos Possani ◽  
...  
Keyword(s):  
Phage Display ◽  
Directed Evolution ◽  
Human Antibodies

Directed Evolution of Bacillus subtilis Lipase A by Use of Enantiomeric Phosphonate Inhibitors: Crystal Structures and Phage Display Selection

ChemBioChem ◽  
2005 ◽  
Vol 7 (1) ◽  
pp. 149-157 ◽  
Author(s):  
Melloney J. Dröge ◽  
Ykelien L. Boersma ◽  
Gertie van Pouderoyen ◽  
Titia E. Vrenken ◽  
Carsten J. Rüggeberg ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Phage Display ◽  
Directed Evolution ◽  
Crystal Structures ◽  
Lipase A

scholarly journals Directed Evolution of the Forkhead-Associated Domain to Generate Anti-Phosphospecific Reagents by Phage Display

2012 ◽  
Vol 424 (1-2) ◽  
pp. 88-103 ◽  
Author(s):  
Kritika Pershad ◽  
Karolina Wypisniak ◽  
Brian K. Kay
Keyword(s):  
Phage Display ◽  
Directed Evolution

Directed Evolution of Highly Homologous Proteins with Different Folds by Phage Display:  Implications for the Protein Folding Code†

Biochemistry ◽  
2005 ◽  
Vol 44 (43) ◽  
pp. 14045-14054 ◽  
Author(s):  
Patrick A. Alexander ◽  
David A. Rozak ◽  
John Orban ◽  
Philip N. Bryan
Keyword(s):  
Protein Folding ◽  
Phage Display ◽  
Directed Evolution ◽  
Homologous Proteins

scholarly journals Affinity maturation of Cry1Aa toxin to the Bombyx mori cadherin‐like receptor by directed evolution based on phage display and biopanning selections of domain II loop 2 mutant toxins

2014 ◽  
Vol 3 (4) ◽  
pp. 568-577 ◽  
Author(s):  
Haruka Endo ◽  
Yuki Kobayashi ◽  
Yasushi Hoshino ◽  
Shiho Tanaka ◽  
Shingo Kikuta ◽  
...  
Keyword(s):  
Phage Display ◽  
Directed Evolution ◽  
Bombyx Mori ◽  
Affinity Maturation ◽  
Loop 2

Phage display as a tool for the directed evolution of enzymes

2003 ◽  
Vol 21 (9) ◽  
pp. 408-414 ◽  
Author(s):  
Ana Fernandez-Gacio ◽  
Marilyne Uguen ◽  
Jacques Fastrez
Keyword(s):  
Phage Display ◽  
Directed Evolution
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