Methods and Strategies for Gene Transfer and Engineering of the Germ Line

2003 ◽  
pp. 121-152
Keyword(s):  
Gene Transfer ◽  
Germ Line

Transgenic Mice: Gene Transfer into the Germ Line

Gene Transfer ◽  
1986 ◽  
pp. 189-221 ◽  
Author(s):  
Shirley M. Tilghman ◽  
Arnold J. Levine
Keyword(s):  
Gene Transfer ◽  
Transgenic Mice ◽  
Germ Line

No evidence of germ-line transmission by adenovirus-mediated gene transfer to mouse testes

2008 ◽  
Vol 89 (5) ◽  
pp. 1448-1454 ◽  
Author(s):  
Yoshiyuki Kojima ◽  
Yutaro Hayashi ◽  
Satoshi Kurokawa ◽  
Kentaro Mizuno ◽  
Shoichi Sasaki ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Germ Line ◽  
Germ Line Transmission

Gene transfer into the Drosophila germ line

Science ◽  
1982 ◽  
Vol 218 (4570) ◽  
pp. 364-365 ◽  
Author(s):  
J. Marx
Keyword(s):  
Gene Transfer ◽  
Germ Line

Correction of murine beta-thalassemia by gene transfer into the germ line

Science ◽  
1986 ◽  
Vol 233 (4769) ◽  
pp. 1192-1194 ◽  
Author(s):  
F Costantini ◽  
K Chada ◽  
J Magram
Keyword(s):  
Gene Transfer ◽  
Germ Line ◽  
Beta Thalassemia

Gene transfer into the mouse germ-line

1982 ◽  
Vol 113 (S1) ◽  
pp. 219-226 ◽  
Author(s):  
Franklin Costantini ◽  
Elizabeth Lacy
Keyword(s):  
Gene Transfer ◽  
Germ Line

scholarly journals Widespread Gene Delivery and Structure-Specific Patterns of Expression in the Brain after Intraventricular Injections of Neonatal Mice with an Adeno-Associated Virus Vector

2001 ◽  
Vol 75 (24) ◽  
pp. 12382-12392 ◽  
Author(s):  
Marco A. Passini ◽  
John H. Wolfe
Keyword(s):  
Gene Transfer ◽  
Viral Vector ◽  
Germ Line ◽  
Cell Infection ◽  
Adeno Associated Virus ◽  
Virus Serotype ◽  
Somatic Gene ◽  
The Central Nervous System ◽  
Somatic Gene Transfer ◽  
Germ Line Transmission

ABSTRACT Developing a system for widespread somatic gene transfer in the central nervous system (CNS) would be beneficial for understanding the global influence of exogenous genes on animal models. We injected an adeno-associated virus serotype 2 (AAV2) vector into the cerebral lateral ventricles at birth and mapped its distribution and transduction pattern from a promoter capable of expression in multiple targets. The injections resulted in structure-specific patterns of expression that were maintained for at least 1 year in most regions, with efficient targeting of some of the major principal neuron layers. The patterns of transduction were explained by circulation of the viral vector in the subarachnoid space via CSF flow, followed by transduction of underlying structures, rather than by progenitor cell infection and subsequent migration. This study demonstrates that gene transfer throughout the CNS can be achieved without germ line transmission and establishes an experimental strategy for introducing genes to somatic cells in a highly predictable manner.

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