Fluorescence Lifetime Imaging: New Microscopy Technologies

2003 ◽  
pp. 139-173 ◽  
Author(s):  
Weiming Yu ◽  
William W. Mantulin ◽  
Enrico Gratton
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Phasor S-FLIM: a new paradigm for fast and robust spectral fluorescence lifetime imaging

2021 ◽  
Author(s):  
Lorenzo Scipioni ◽  
Alessandro Rossetta ◽  
Giulia Tedeschi ◽  
Enrico Gratton
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
New Paradigm ◽  
Lifetime Imaging

Interrogation of the liver during regeneration by fluorescence lifetime imaging and mass spectrometry

2021 ◽  
pp. 1-1
Author(s):  
Svetlana Rodimova ◽  
Daria Kuznetsova ◽  
Nikolai Bobrov ◽  
Alexander Gulin ◽  
Dmitry Reunov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Fluorescence-Lifetime Imaging of DNA–Dye Interactions within Continuous-Flow Microfluidic Systems

2007 ◽  
Vol 119 (13) ◽  
pp. 2278-2281 ◽  
Author(s):  
Richard K. P. Benninger ◽  
Oliver Hofmann ◽  
Björn Önfelt ◽  
Ian Munro ◽  
Chris Dunsby ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Continuous Flow ◽  
Fluorescence Lifetime Imaging ◽  
Microfluidic Systems ◽  
Lifetime Imaging

scholarly journals Investigation of the Membrane Fluidity Regulation of Fatty Acid Intracellular Distribution by Fluorescence Lifetime Imaging of Novel Polarity Sensitive Fluorescent Derivatives

2021 ◽  
Vol 22 (6) ◽  
pp. 3106
Author(s):  
Giada Bianchetti ◽  
Salome Azoulay-Ginsburg ◽  
Nimrod Yosef Keshet-Levy ◽  
Aviv Malka ◽  
Sofia Zilber ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Membrane Fluidity ◽  
Fluorescence Lifetime ◽  
Intracellular Distribution ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Fatty Acid Derivatives ◽  
Polarity Sensitive

Free fatty acids are essential structural components of the cell, and their intracellular distribution and effects on membrane organelles have crucial roles in regulating the metabolism, development, and cell cycle of most cell types. Here we engineered novel fluorescent, polarity-sensitive fatty acid derivatives, with the fatty acid aliphatic chain of increasing length (from 12 to 18 carbons). As in the laurdan probe, the lipophilic acyl tail is connected to the environmentally sensitive dimethylaminonaphthalene moiety. The fluorescence lifetime imaging analysis allowed us to monitor the intracellular distribution of the free fatty acids within the cell, and to simultaneously examine how the fluidity and the microviscosity of the membrane environment influence their localization. Each of these probes can thus be used to investigate the membrane fluidity regulation of the correspondent fatty acid intracellular distribution. We observed that, in PC-12 cells, fluorescent sensitive fatty acid derivatives with increased chain length compartmentalize more preferentially in the fluid regions, characterized by a low microviscosity. Moreover, fatty acid derivatives with the longest chain compartmentalize in lipid droplets and lysosomes with characteristic lifetimes, thus making these probes a promising tool for monitoring lipophagy and related events.

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