The Glycal Assembly Method on Solid Supports: Synthesis of Oligosaccharides and Glycoconjugates

2003 ◽  
pp. 15-40 ◽  
Author(s):  
Pier F. Cirillo ◽  
Samuel J. Danishefsky
Keyword(s):  
Solid Supports ◽  
Assembly Method

Dendrimer-Modified Solid Supports: Nanostructured Materials with Potential Drug Allergy Diagnostic Applications

2012 ◽  
Vol 19 (29) ◽  
pp. 4942-4954 ◽  
Author(s):  
A. J. Ruiz-Sanchez ◽  
M. I. Montanez ◽  
C. Mayorga ◽  
M. J. Torres ◽  
N. S. Kehr ◽  
...  
Keyword(s):  
Nanostructured Materials ◽  
Drug Allergy ◽  
Potential Drug ◽  
Solid Supports ◽  
Diagnostic Applications

Supported protic acid-catalyzed synthesis of 2,3-disubstituted thiazolidin-4-ones: enhancement of the catalytic potential of protic acid by adsorption on solid supports

2013 ◽  
Vol 15 (10) ◽  
pp. 2872 ◽  
Author(s):  
Dinesh Kumar ◽  
Mukesh Sonawane ◽  
Brahmam Pujala ◽  
Varun K. Jain ◽  
Srikant Bhagat ◽  
...  
Keyword(s):  
Solid Supports ◽  
Catalytic Potential ◽  
Acid Catalyzed

Stabilization of bilayer lipid membranes on solid supports by trehalose

1996 ◽  
Vol 39 (2) ◽  
pp. 299-302 ◽  
Author(s):  
T. Hianik ◽  
J. Dlugopolsky ◽  
M. Gyeppessova ◽  
B. Sivak ◽  
H.T. Tien ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Solid Supports ◽  
Bilayer Lipid

scholarly journals Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Mohamed Ramadan ◽  
Muna Alariqi ◽  
Yizan Ma ◽  
Yanlong Li ◽  
Zhenping Liu ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Sequence Similarity ◽  
High Specificity ◽  
Transformation Method ◽  
High Sequence Similarity ◽  
Single Experiment ◽  
Next Generation Sequencing Technology ◽  
Cotton Transformation ◽  
Assembly Method ◽  
Multiple Genes

Abstract Background Upland cotton (Gossypium hirsutum), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic and functional analyses extremely challenging. The recent accessibility of CRISPR/Cas9 tool provides the ability to modify targeted locus efficiently in various complicated plant genomes. However, current cotton transformation method targeting one gene requires a complicated, long and laborious regeneration process. Hence, optimizing strategy that targeting multiple genes is of great value in cotton functional genomics and genetic engineering. Results To target multiple genes in a single experiment, 112 plant development-related genes were knocked out via optimized CRISPR/Cas9 system. We optimized the key steps of pooled sgRNAs assembly method by which 116 sgRNAs pooled together into 4 groups (each group consisted of 29 sgRNAs). Each group of sgRNAs was compiled in one PCR reaction which subsequently went through one round of vector construction, transformation, sgRNAs identification and also one round of genetic transformation. Through the genetic transformation mediated Agrobacterium, we successfully generated more than 800 plants. For mutants identification, Next Generation Sequencing technology has been used and results showed that all generated plants were positive and all targeted genes were covered. Interestingly, among all the transgenic plants, 85% harbored a single sgRNA insertion, 9% two insertions, 3% three different sgRNAs insertions, 2.5% mutated sgRNAs. These plants with different targeted sgRNAs exhibited numerous combinations of phenotypes in plant flowering tissues. Conclusion All targeted genes were successfully edited with high specificity. Our pooled sgRNAs assembly offers a simple, fast and efficient method/strategy to target multiple genes in one time and surely accelerated the study of genes function in cotton.

scholarly journals Controlled growth of ultrafine metal nanoparticles mediated by solid supports

2021 ◽  
Vol 3 (7) ◽  
pp. 1865-1886
Author(s):  
Hongyin Hu ◽  
Shuanglong Lu ◽  
Ting Li ◽  
Yue Zhang ◽  
Chenxi Guo ◽  
...  
Keyword(s):  
Metal Nanoparticles ◽  
Catalytic Properties ◽  
Structural Features ◽  
Controlled Growth ◽  
Solid Supports ◽  
Ultrafine Metal ◽  
Different Types

This article reviews the controlled growth of UMNPs mediated by different types of solid supports and their catalytic properties. The importance of certain structural features of the supports is also discussed.

scholarly journals Design of Self-Reconfigurable Multiarm Robot Mechanism Based on Deployable Kinematic Chains

2020 ◽  
Vol 33 (1) ◽  
Author(s):  
Fu-Qun Zhao ◽  
Sheng Guo ◽  
Hai-Jun Su ◽  
Hai-Bo Qu ◽  
Ya-Qiong Chen
Keyword(s):  
Parallel Mechanism ◽  
Parallel Mechanisms ◽  
Kinematic Chains ◽  
Assembly Method ◽  
Operation Modes ◽  
Object Based ◽  
Switch Operation ◽  
Storage Area ◽  
Mechanism Theory

Abstract As the structures of multiarm robots are serially arranged, the packaging and transportation of these robots are often inconvenient. The ability of these robots to operate objects must also be improved. Addressing this issue, this paper presents a type of multiarm robot that can be adequately folded into a designed area. The robot can achieve different operation modes by combining different arms and objects. First, deployable kinematic chains (DKCs) are designed, which can be folded into a designated area and be used as an arm structure in the multiarm robot mechanism. The strategy of a platform for storing DKCs is proposed. Based on the restrictions in the storage area and the characteristics of parallel mechanisms, a class of DKCs, called base assembly library, is obtained. Subsequently, an assembly method for the synthesis of the multiarm robot mechanism is proposed, which can be formed by the connection of a multiarm robot mechanism with an operation object based on a parallel mechanism structure. The formed parallel mechanism can achieve a reconfigurable characteristic when different DKCs connect to the operation object. Using this method, two types of multiarm robot mechanisms with four DKCs that can switch operation modes to perform different tasks through autonomous combination and release operation is proposed. The obtained mechanisms have observable advantages when compared with the traditional mechanisms, including optimizing the occupied volume during transportation and using parallel mechanism theory to analyze the switching of operation modes.

scholarly journals Wafer-Scale Fabrication and Assembly Method of Multichannel Microelectrode Arrays for ECoG Application

Electronics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 316
Author(s):  
Cong Wang ◽  
Yu-Chen Wei ◽  
Ho-Kun Sung ◽  
Alok Kumar ◽  
Zhong-Liang Zhou ◽  
...  
Keyword(s):  
Printed Circuit Boards ◽  
Electrochemical Impedance ◽  
Microelectrode Arrays ◽  
Absence Seizure ◽  
Curing Conditions ◽  
Wafer Scale ◽  
In Vivo Test ◽  
Assembly Method ◽  
Signal Recording

High density electrocorticography (ECoG)-based microelectrode arrays (MEAs) are fabricated to timely record the neural activities to provide the fundamental understanding in neuroscience and biomedical engineering. This paper aims to introduce a device-based concept and wafer-scale fabrication process for MEAs. Flexible and biocompatible polyimide is applied on MEAs to bear all possible stress and strain. Detailed fabrication key techniques, including surface treatment, polyimide stability measurement, evaporation process, and curing conditions, have been discussed thoroughly. Moreover, the fabricated polyimide-based MEAs are surface-mounted on well-packaged printed circuit boards (PCBs) via a slot-type connector without any additional wire bonding to make the signal recording process easier. An absence seizure was recorded during the in vivo test, which shows the availability of signal recording based on the presented MEAs. The proposed MEAs could be remained at the skull, while the connector and PCBs can be disassembled apart. Therefore, the testing sample will get less suffering. To verify the robustness of the fabricated MEAs, the impedance properties were characterized using electrochemical impedance spectroscopy. The measured results indicate an average impedance of 12.3 ± 0.675 kΩ at 1 kHz. In total, 10 groups of MEAs were sample tested, and over 90% of the total 60 channels per 1-MEAs operated efficiently.

scholarly journals Multistage Asymmetric Rotors Coaxial Measurement Stacking Method Based on Minimization of Exciting Force

Symmetry ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1054
Author(s):  
Yongmeng Liu ◽  
Yingjie Mei ◽  
Chuanzhi Sun ◽  
Pinghuan Xiao ◽  
Ruirui Li ◽  
...  
Keyword(s):  
Vibration Amplitude ◽  
High Speed ◽  
Geometric Error ◽  
Exciting Force ◽  
Propagation Model ◽  
Assembly Method ◽  
Geometric Center ◽  
Asymmetric Rotor ◽  
Rotor Equipment ◽  
Geometric Error Measurement

The unbalanced exciting force of high-speed rotary asymmetric rotor equipment is the main factor causing rotor vibration. In order to effectively suppress the vibration of the asymmetric rotor equipment, the paper establishes a multistage asymmetric rotor coaxial measurement stacking method that minimizes the exciting force. By analyzing the propagation process of the centroid of the multistage asymmetric rotor assembly and analyzing the relationship between the geometric center and the centroid of a single asymmetric rotor, a multistage asymmetric unbalanced rotor propagation model based on geometric center stacking is established. The genetic algorithm is used to optimize the unbalance of the multistage asymmetric rotors. Combined with the vibration principle under the exciting force, the vibration amplitude of the left bearing at different rotation speeds under the minimization of the exciting force and the random assembly phase is analyzed. Finally, the experimental asymmetric rotors are dynamically measured, combined with the asymmetric rotors’ geometric error measurement experiment. The experimental results confirm that the vibration amplitude of the assembly phase with the minimum exciting force is smaller than the vibration amplitude under the random assembly phase at three-speed modes, and the optimization rate reached 73.2% at 9000 rpm, which proves the effectiveness of the assembly method in minimizing the exciting force.

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