Preparation of Equipment up to Sample Injection

2006 ◽  
pp. 58-72
Keyword(s):  
Sample Injection

Determination of trace concentrations of copper by FIA-FAAS after preconcentration on chelating sorbents

1989 ◽  
Vol 54 (7) ◽  
pp. 1785-1794 ◽  
Author(s):  
Vlastimil Kubáň ◽  
Josef Komárek ◽  
Zbyněk Zdráhal
Keyword(s):  
Sampling Frequency ◽  
Copper Determination ◽  
Flow Rates ◽  
Sample Injection ◽  
Injection Flow ◽  
Chelating Sorbents ◽  
Set Up ◽  
Trace Concentrations ◽  
Better Than

A FIA-FAAS apparatus containing a six-channel sorption equipment with five 3 x 26 mm microcolumns packed with Spheron Oxin 1 000, Ostsorb Oxin and Ostsorb DTTA was set up. Combined with sorption from 0.002M acetate buffer at pH 4.2 and desorption with 2M-HCl, copper can be determined at concentrations up to 100, 150 and 200 μg l-1, respectively. For sample and eluent flow rates of 5.0 and 4.0 ml min-1, respectively, and a sample injection time of 5 min, the limit of copper determination is LQ = 0.3 μg l-1, repeatability sr is better than 2% and recovery is R = 100 ± 2%. The enrichment factor is on the order of 102 and is a linear function of time (volume) of sample injection up to 5 min and of the sample injection flow rate up to 11 ml min-1 for Spheron Oxin 1 000 and Ostsorb DTTA. For times of sorption of 60 and 300 s, the sampling frequency is 70 and 35 samples/h, respectively. The parameters of the FIA-FAAS determination (acetylene-air flame) are comparable to or better than those achieved by ETA AAS. The method was applied to the determination of traces of copper in high-purity water.

Photon-counting microcolorimeter with 40-nl cuvette

1991 ◽  
Vol 261 (1) ◽  
pp. F203-F206
Author(s):  
T. L. Pallone
Keyword(s):  
Lower Limit ◽  
Plasma Volume ◽  
Fiber Optic ◽  
Limit Of Detection ◽  
Photon Counting ◽  
Injection Port ◽  
Photon Counter ◽  
Sample Injection ◽  
Lowry Assay

A colorimeter with a 40-nl cuvette has been constructed. The wall of standard capillary glass was perforated to produce a sample injection port. A section of the capillary glass was drawn to a length of 1/2 cm and 80 microns ID by heating on a microforge. This produced a cuvette volume of approximately 40 nl. Two fiber-optic filaments, 80 microns in diameter, were fixed into the cuvette to transmit and receive light from the sample. The output of the colorimeter was measured with a microscope photon-counting detection assembly. It has been shown that the colorimeter enables a reduction of the plasma volume requirements of the Lowry microprotein assay from several nanoliters to 60 pl. The linearity and reproducibility of the microcolorimeter when used with the Lowry assay has been verified. The colorimeter output is several orders of magnitude above the lower limit of detection of the photon counter.

PDMS–glass serpentine microchannel chip for time domain PCR with bubble suppression in sample injection

2007 ◽  
Vol 17 (9) ◽  
pp. 1810-1817 ◽  
Author(s):  
Chul-Ho Cho ◽  
Woong Cho ◽  
Yoomin Ahn ◽  
Seung-Yong Hwang
Keyword(s):  
Time Domain ◽  
Sample Injection
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