Regulatory Interaction between CFTR and the SLC26 Transporters

2008 ◽  
pp. 177-192 ◽  
Author(s):  
Nikolay Shcheynikov ◽  
Shigeru B. H. Ko ◽  
Weizhong Zeng ◽  
Joo Young Choi ◽  
Michael R. Dorwart ◽  
...  
Keyword(s):  
Regulatory Interaction

Introduction: Governing Regulatory Interaction: the Normative Question

2006 ◽  
Vol 12 (4) ◽  
pp. 431-439 ◽  
Author(s):  
Viktor Mayer-Schonberger ◽  
Alexander Somek
Keyword(s):  
Regulatory Interaction ◽  
Normative Question ◽  
The Normative Question

Complementation and regulatory interaction between two cloned fimbrial gene clusters of Escherichia coli strain KS71

1985 ◽  
Vol 200 (1) ◽  
pp. 60-64 ◽  
Author(s):  
Mikael Rhen ◽  
Vuokko Väisänen-Rhen ◽  
Auli Pere ◽  
Timo K. Korhonen
Keyword(s):  
Escherichia Coli ◽  
Gene Clusters ◽  
Coli Strain ◽  
Regulatory Interaction

scholarly journals Mechanism of N-Wasp Activation by Cdc42 and Phosphatidylinositol 4,5-Bisphosphate

2000 ◽  
Vol 150 (6) ◽  
pp. 1299-1310 ◽  
Author(s):  
Rajat Rohatgi ◽  
Hsin-yi Henry Ho ◽  
Marc W. Kirschner
Keyword(s):  
Binding Site ◽  
Physical Interaction ◽  
Regulatory Interaction ◽  
Actin Nucleation ◽  
Sequence Element ◽  
Effector Domain ◽  
Nucleation Activity ◽  
Syndrome Protein ◽  
Upstream Regulators ◽  
Terminal Effector

Neuronal Wiskott-Aldrich Syndrome protein (N-WASP) transmits signals from Cdc42 to the nucleation of actin filaments by Arp2/3 complex. Although full-length N-WASP is a weak activator of Arp2/3 complex, its activity can be enhanced by upstream regulators such as Cdc42 and PI(4,5)P2. We dissected this activation reaction and found that the previously described physical interaction between the NH2-terminal domain and the COOH-terminal effector domain of N-WASP is a regulatory interaction because it can inhibit the actin nucleation activity of the effector domain by occluding the Arp2/3 binding site. This interaction between the NH2- and COOH termini must be intramolecular because in solution N-WASP is a monomer. Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) influences the activity of N-WASP through a conserved basic sequence element located near the Cdc42 binding site rather than through the WASp homology domain 1. Like Cdc42, PI(4,5)P2 reduces the affinity between the NH2- and COOH termini of the molecule. The use of a mutant N-WASP molecule lacking this basic stretch allowed us to delineate a signaling pathway in Xenopus extracts leading from PI(4,5)P2 to actin nucleation through Cdc42, N-WASP, and Arp2/3 complex. In this pathway, PI(4,5)P2 serves two functions: first, as an activator of N-WASP; and second, as an indirect activator of Cdc42.

scholarly journals Structural basis for the regulatory interaction of the methylglyoxal synthase MgsA with the carbon flux regulator Crh inBacillus subtilis

2018 ◽  
Vol 293 (16) ◽  
pp. 5781-5792 ◽  
Author(s):  
Achim Dickmanns ◽  
Christopher P. Zschiedrich ◽  
Johannes Arens ◽  
Iwan Parfentev ◽  
Jan Gundlach ◽  
...  
Keyword(s):  
Carbon Flux ◽  
Structural Basis ◽  
Regulatory Interaction ◽  
Methylglyoxal Synthase

scholarly journals Coilin enhances phosphorylation and stability of DGCR8 and promotes miRNA biogenesis

2021 ◽  
pp. mbc.E21-05-0225
Author(s):  
Katheryn E. Lett ◽  
Madelyn K. Logan ◽  
Douglas M. McLaurin ◽  
Michael D. Hebert
Keyword(s):  
Noncoding Rnas ◽  
Mature Mirnas ◽  
Cajal Body ◽  
Mirna Biogenesis ◽  
Regulatory Interaction ◽  
Small Noncoding Rnas ◽  
Control Gene Expression ◽  
Processing Steps ◽  
Posttranscriptional Level

MicroRNAs (miRNAs) are ∼22 nt small noncoding RNAs that control gene expression at the posttranscriptional level through translational inhibition and destabilization of their target mRNAs. The biogenesis of miRNAs involves a series of processing steps beginning with cropping of the primary miRNA transcript by the Microprocessor complex, which is comprised of Drosha and DGCR8. Here we report a novel regulatory interaction between the Microprocessor components and coilin, the Cajal Body (CB) marker protein. Coilin knockdown causes alterations in the level of primary and mature miRNAs, let-7a and miR-34a, and their miRNA targets, HMGA2 and Notch1, respectively. We also found that coilin knockdown affects the levels of DGCR8 and Drosha in cells with (HeLa) and without (WI-38) CBs. To further explore the role of coilin in miRNA biogenesis, we conducted a series of co-immunoprecipitation experiments using coilin and DGCR8 constructs, which revealed that coilin and DGCR8 can form a complex. Additionally, our results indicate that phosphorylation of DGCR8, which has been shown to increase protein stability, is impacted by coilin knockdown. Collectively, our results implicate coilin as a member of the regulatory network governing miRNA biogenesis.

A CYC-RAD-DIV-DRIF interaction likely pre-dates the origin of floral monosymmetry in Lamiales

2021 ◽  
Author(s):  
Aniket Sengupta ◽  
Lena C. Hileman
Keyword(s):  
Evolutionary Biology ◽  
De Novo ◽  
Tomato Fruit ◽  
Regulatory Interaction ◽  
En Bloc ◽  
Regulatory Interactions ◽  
Developmental Program ◽  
Novel Traits ◽  
Carpel Development ◽  
Outstanding Question

Abstract BackgroundAn outstanding question in evolutionary biology is how genetic interactions defining novel traits evolve. They may evolve either by de novo assembly of previously non-interacting genes or by en bloc co-option of interactions from other functions. We tested these hypotheses in the context of a novel phenotype—Lamiales flower monosymmetry—defined by a developmental program that relies on regulatory interaction among CYCLOIDEA , RADIALIS , DIVARICATA , and DRIF gene products. In Antirrhinum majus (snapdragon), representing Lamiales, we tested whether components of this program likely function beyond their previously known role in petal and stamen development. In Solanum lycopersicum (tomato), representing Solanales which diverged from Lamiales before the origin of Lamiales floral monosymmetry, we additionally tested for regulatory interactions in this program. ResultsWe found that RADIALIS , DIVARICATA , and DRIF are expressed in snapdragon ovaries and developing fruit, similar to their homologs during tomato fruit development. Additionally, we found that a tomato CYCLOIDEA ortholog positively regulates a tomato RADIALIS ortholog. ConclusionOur results provide preliminary support to the hypothesis that the developmental program defining floral monosymmetry in Lamiales was co-opted en bloc from a function in carpel development. This expands our understanding of novel trait evolution facilitated by co-option of existing regulatory interactions.

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