Dysglobulinemia and iron in plasma cells. Ferrokinetics and electron microscopy

1968 ◽  
Vol 121 (3) ◽  
pp. 284-287 ◽  
Author(s):  
R. G. Lerner
Keyword(s):  
Electron Microscopy ◽  
Plasma Cells

The Ultrastructure of Lymphocytic Hypophysitis

1981 ◽  
Vol 39 ◽  
pp. 466-467
Author(s):  
S.L. Asa ◽  
K. Kovacs ◽  
J. M. Bilbao ◽  
R. G. Josse ◽  
K. Kreines
Keyword(s):  
Electron Microscopy ◽  
Plasma Cells ◽  
Secretory Granules ◽  
Sella Turcica ◽  
Uranyl Acetate ◽  
Lymphocytic Hypophysitis ◽  
Pituitary Cells ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Mass Lesions

Seven cases of lymphocytic hypophysitis in women have been reported previously in association with various degrees of hypopituitarism. We report two pregnant patients who presented with mass lesions of the sella turcica, clinically mimicking pituitary adenoma. However, pathologic examination revealed extensive infiltration of the anterior pituitary by lymphocytes and plasma cells with destruction of the gland. To our knowledge, the ultrastructural features of lymphocytic hypophysitis have not been studied so far.For transmission electron microscopy, tissue from surgical specimens was fixed in glutaraldehyde, postfixed in OsO4, dehydrated and embedded in epoxy-resin. Ultrathin sections were stained with uranyl acetate and lead citrate and examined with a Philips 300 electron microscope.Electron microscopy revealed adenohypophysial cells of all types exhibiting varying degrees of injury. In the areas of most dense inflammatory cell infiltration pituitary cells contained large lysosomal bodies fusing with secretory granules (Fig. 1), as well as increased numbers of swollen mitochondria, indicating oncocytic transformation (Fig. 2).

scholarly journals ELECTRON MICROSCOPY OF PLASMA-CELL TUMORS OF THE MOUSE

1961 ◽  
Vol 9 (2) ◽  
pp. 353-368 ◽  
Author(s):  
D. F. Parsons ◽  
E. B. Darden ◽  
D. L. Lindsley ◽  
Guthrie T. Pratt
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Plasma Cell ◽  
Electron Microscope Study ◽  
Plasma Cells ◽  
Granular Body ◽  
Virus Particles ◽  
Cytoplasmic Structure ◽  
Large Numbers ◽  
C3h Mice

An electron microscope study was made of a series of transplanted MPC-1 plasma-cell tumors carried by BALB/c mice. Large numbers of particles similar in morphology to virus particles were present inside the endoplasmic reticulum of tumor plasma cells. Very few particles were seen outside the cells or in ultracentrifuged preparations of the plasma or ascites fluid. In very early tumors particles were occasionally seen free in the cytoplasm adjacent to finely granular material. In general, the distribution of these particles inside endoplasmic reticulum is similar in early and late tumors. A few transplanted X5563 tumors of C3H mice were also examined. Large numbers of particles were found in the region of the Golgi apparatus in late X5663 tumors. A newly described cytoplasmic structure of plasma cells, here called a "granular body," appears to be associated with the formation of the particles. Particles present in MPC-1 tumors are exclusively of a doughnut form, whereas some of those in the inclusions of the late X5563 tumors show a dense center. Normal plasma cells, produced by inoculation of a modified Freund adjuvant into BALB/c mice. have been compared morphologically with tumor plasma cells of both tumor lines.

scholarly journals Demonstration of a Cytoplasmic Structure in Plasma Cells

Blood ◽  
1953 ◽  
Vol 8 (10) ◽  
pp. 916-922 ◽  
Author(s):  
H. BRAUNSTEINER ◽  
K. FELLINGER ◽  
F. PAKESCH
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Plasma Cells ◽  
Filamentous Structure ◽  
Cytoplasmic Structure

Abstract In normal and pathologic plasma cells of the bone marrow, a specific filamentous structure of the cytoplasm has been revealed by electron microscopy. The significance of this structure is briefly discussed.

scholarly journals Ultrastructural and Immunofluorescence Studies of the Cells Associated With µ-Chain Disease

Blood ◽  
1971 ◽  
Vol 37 (3) ◽  
pp. 257-271 ◽  
Author(s):  
DOROTHEA ZUCKER-FRANKLIN ◽  
EDWARD C. FRANKLIN
Keyword(s):  
Electron Microscopy ◽  
Secretory Pathway ◽  
Plasma Cells ◽  
Bone Marrow Cells ◽  
Light Chains ◽  
Antigenic Determinants ◽  
Protein Fragments ◽  
Fluorescence And Electron Microscopy ◽  
Golgi Zone ◽  
Heavy Chain Disease

Abstract Fluorescence and electron microscopy studies were carried out on the blood and bone marrow cells of the first patient observed to have µ-chain disease. Since previously reported cases of γ and α heavy-chain disease did not elaborate any light chains, it was of interest to determine whether both the µ-chain fragments and the κ chains found in the serum of this patient originated in the same cell or whether mutations had affected two different clones. The use of rhodamine-conjugated and fluorescein-conjugated antisera to heavy and light chains respectively established that both antigenic determinants were present in the same cell. On electron microscopy, the plasma cells showed large vacuoles which appeared to form in the vicinity of the Golgi zone and frequently extended to the surface of the cell. It is postulated that the structural defect in the µ chain interferes with proper assembly of the gamma-globulin molecule. This in turn may preclude transport via the normal secretory pathway. The accumulated protein fragments may be released by a process of limited cytolysis.

scholarly journals ULTRASTRUCTURAL LOCALIZATION OF ANTIBODY IN DIFFERENTIATING PLASMA CELLS

1968 ◽  
Vol 127 (1) ◽  
pp. 109-118 ◽  
Author(s):  
Elizabeth H. Leduc ◽  
Stratis Avrameas ◽  
Michel Bouteille
Keyword(s):  
Electron Microscopy ◽  
Golgi Apparatus ◽  
Horseradish Peroxidase ◽  
Peroxidase Activity ◽  
Plasma Cells ◽  
Ultrastructural Localization ◽  
Fixed Tissue ◽  
Plasmacytic Differentiation ◽  
Perinuclear Space ◽  
Large Spherical

Antibody was localized by electron microscopy within differentiating and mature plasma cells of the spleens of hyperimmunized rabbits. Horseradish peroxidase was used as antigen. Intracellular antibody to peroxidase was revealed in glutaraldehyde-fixed tissue by coupling it with its antigen and then revealing the sites of peroxidase activity cytochemically. Antibody first appears in the perinuclear space of hemocytoblasts where it persists through differentiation into immature plasma cells, but it disappears from this site in mature plasma cells. Concomitant with the development of the ergastoplasm, antibody accumulates in many but not all of its cisternae. Antibody is present in the lamellar portion of the Golgi apparatus in all phases of plasmacytic differentiation. Mature plasma cells exhibit two types of antibody distribution, a concentration into large spherical intracisternal granules or an overflowing into all parts of the cytoplasm.

scholarly journals STUDIES ON ANTIBODY-PRODUCING CELLS

1970 ◽  
Vol 132 (3) ◽  
pp. 448-474 ◽  
Author(s):  
Fred G. Gudat ◽  
T. N. Harris ◽  
Susanna Harris ◽  
Klaus Hummeler
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Lymph Node ◽  
Blood Cells ◽  
Plasma Cells ◽  
Great Majority ◽  
The Other ◽  
Nuclear Chromatin ◽  
Parallel Orientation ◽  
Transitional Cells

Antibody-bearing cells of spleen and lymph node of the mouse and rabbit detected by rosette formation with the antigenic red blood cells were collected by micropipet and studied by electron microscopy. More than 300 such cells were examined. In the lymph nodes, rosette-forming cells were all in the lymphocytic and plasmacytic categories. In cells of the mouse spleen, macrophages were also found among the RFC, especially in the later days after immunization. The great majority of the RFC, 70–100%, were of the lymphocytic category. These included small, medium, and large lymphocytes with fine gradations of differentiation, and blast forms with little heterochromatin. The endoplasmic reticulum of these cells occurred in short, very narrow pieces, usually in contact with a mitochondrion. The cells of the plasmacytic category also showed fine gradations from plasmablasts to typical mature plasma cells. Plaque-forming cells of mouse and rabbit were also collected by micropipet. Of 162 such cells, fine gradations were also found throughout the lymphocytic and plasmacytic categories, but in this case the great majority were in the plasmacytic group, and more plasma cells showed amorphous nuclear chromatin. Among antibody-forming cells detected by both reactions, some of the more highly differentiated large lymphocytes contained ER which differed from that in the other large lymphocytes in that the channels were slightly and variably distended, with deposition of some precipitate, and with some tendency to a more nearly parallel orientation of the few channels seen. These were considered transitional cells. Of 10 RFC found in mitosis, all were in the lymphocytic category, in various stages of differentiation, the most advanced of which (in 2 of the 10 cells) was that of the transitional lymphocyte described here. Cells producing plaques facilitated by antisera vs. IgG of the mouse or rabbit (7S) showed the same distribution between cell categories and the same fine gradations as the direct (19S) PFC. Cells producing rosettes which were resistant to lysis in the presence of complement, and were thus presumably producing 7S antibody, showed a distribution similar to that found generally with rosette-forming cells, approximately 80–90% in the lymphocytic category.

scholarly journals Phagocytized Plasma Cells in Mouse Spleen Observed by Light and Electron Microscopy

Blood ◽  
1964 ◽  
Vol 24 (4) ◽  
pp. 432-442 ◽  
Author(s):  
D. C. SWARTZENDRUBER
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Microscopic Study ◽  
Plasma Cells ◽  
Light And Electron Microscopy ◽  
Mouse Spleen ◽  
White Pulp ◽  
Electron Microscopic ◽  
Single Intravenous Injection ◽  
Rat Bone

Abstract A correlative light and electron microscopic study is reported of pyknotic, phagocytized plasma cells in the mouse spleen following a single intravenous injection of sheep erythrocytes or rat bone marrow. Phagocytized plasma cells were observed in both red and white pulp between 3 and 7 days after antigen injection. A few mature plasma cells and plasma cells with Russel bodies were found 10 days after stimulation with no evidence of recognizable phagocytized plasma cells. Phagocytosis clearly plays some role in the removal of plasma cells from the spleen under the conditions employed in this study, although the possibility that significant numbers of plasmacytes migrate out of the spleen is considered. A correlation of phagocytized plasma cells with the log phase of antibody appearance in the circulation is indicated.

scholarly journals Electron Microscopic Localization of Russell Bodies in the Human Plasma Cell

Blood ◽  
1960 ◽  
Vol 16 (3) ◽  
pp. 1307-1312 ◽  
Author(s):  
RONALD A. WELSH
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Human Plasma ◽  
Plasma Cell ◽  
Plasma Cells ◽  
Electron Microscopic ◽  
Degenerative Process ◽  
Microscopic Localization ◽  
Electron Microscopic Localization

Abstract The location of Russell bodies in the human plasma cell was shown by electron microscopy to be within the intracisternal space of the endoplasmic reticulum. The significance of this finding was discussed from the standpoint of possible intracellular function of the endoplasmic reticulum. The appearance of the affected plasma cells tended to negate a degenerative process, and the suggestion was offered that the Russell body results from a condensation of intracisternal secretion.

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